Product Name :
Collagen Type III, rat

Description :
| Units 0.1 ml | Host Rabbit | Application ELISA Immunofluoresence Immunohistochemistry (paraffin) Radioimmunoassay

Background :
Immunogen: Purified collagen type III from rat skin

Source :
Type III collagen is an alpha1(III)-trimer, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and vitreous body. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Rat collagen type III 100%, rat collagen I <

Product :
affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added! Purification Method: affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added! Secondary Reagents: Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:FITC (Art. No. FI-1000) or anti-rabbit IgG:DyLight488 (Art. No. DI-1488). Concentration: app. 1 mg/ml

Specificity :
Species Reactivity: Rat, cross-reaction with mouse

Applications :
IHC(P), IFA, ELISA, RIA Incubation Time: IHC(P) 60 min at RT or 2-8°C over night Working Concentration: (purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:500, ELISA ? 1:200 (OD ? 500) Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Positive Control: Rat skin or liver

Storage :
-20°C

Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References :
1. Bouvier M., Couble M.L., Hartmann D.J., Magloire H. (1991) Isolation and characterization of rat alveolar bone cells. Cell Mol. Biol. 37, 509-517.» 2. Breen E., Falco V.M., Absher M., Cutroneo K.R. (1990) Subpopulations of rat lung fibroblasts with different amounts of type I and type III collagen mRNAs. J. Biol. Chem. 265, 6286-6290. 3. Vialle-Presles M.J., Hartmann D.J., Franc S., Herbage D. (1989) Immunohistochemical study of the biological fate of a subcutaneous bovine collagen implant in rat. Histochemistry 91, 177-184. 4. Al Adnani M.S. (1989) Differential immunohistochemical localization of cytokeratins and collagen types I and III in experimentally-induced cirrhosis. J. Pathol. 159, 151-158.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
Related websites: https://www.medchemexpress.com/antibodies.html
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