Name :
Biotinylated Human 2B4 / CD244 / SLAMF4 Protein, Avitag™,His Tag (recommended for biopanning)
Background :
Natural killer cell receptor 2B4 is also known as NK cell type I receptor protein 2B4 (NKR2B4 or h2B4), SLAM family member 4 (SLAMF4), Signaling lymphocytic activation molecule 4, CD antigen CD244. NKR2B4 / CD244 contains two Ig-like (immunoglobulin-like) domains. CD244 is expressed in spleen, PBL, followed by lung, liver, testis and small intestine. CD244 interacts with CD48. Following phosphorylation, CD244 is able to recruit PTPN11/SHP-2 and SH2D1A/SAP. SLAMF4 modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family.
Biological Activity :
Immobilized Biotinylated Human 2B4 Protein, Avitag,His Tag (Cat. No. 2B4-H82E9) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Human CD48, Fc Tag (Cat. No. BC1-H5255) with a linear range of 0.3-5 ng/mL (QC tested).
Species :
Source :
Biotinylated Human 2B4 Protein, Avitag,His Tag (2B4-H82E9) is expressed from human 293 cells (HEK293). It contains AA Cys 22 – Arg 221 (Accession # Q9BZW8-2 ).
Tag :
Synonyms :
（Synonym）CD244,2B4,SLAMF4,NKR2B4,NAIL,h2B4
Purity :
（Purity）>90% as determined by SDS-PAGE.
Storage and Stability :
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin Level :
（Endotoxin）Less than 1.0 EU per μg by the LAL method.
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Protein Structure :
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag
Refactoring Approach :
Please see Certificate of Analysis for specific instructions.
Protein Labeling :
Passed as determined by the HABA assay / binding ELISA.
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