Product Name :
Human Alkaline phosphatase (Intestinal type) Protein
EN
IAP; Akp2; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; HOPS; MGC161443; TNAP; TNSALP
Description:
| Human Alkaline phosphatase (Intestinal type) /ALPI Protein with the N-His-Flag Tag |Source: The reco|>95% by SEC-HPLC and SDS-|Less than 1 EU per ug of protein as determined by LAL method. |Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |Reconstitution: It is recommended to reconstitute the lyophilized reco|The recombinant Human Alkaline phosphatase (Intestinal type) /ALPI Protein is shipped with ice |Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution.
Source:
The recombinant Human Alkaline phosphatase (Intestinal type) /ALPI Protein, (amino acids Val20-Asp503) fused with a His-Flag tag at the N-terminus, was produced in HEK 293 cells.
Molecular Weight :
The recombinant Human Alkaline phosphatase (Intestinal type) /ALPI Protein with the His-Flag tag at the N-terminus has a predicted MW of 54.5 kDa. Due to glycosylation, the protein migrates to 63-73 kDa based on Tris-Bis PAGE result.
Activity:
The affinity constant of 1.58 uM as determined in SPR assay (Biacore T200).
Purity :
>95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin:
LevelLess than 1 EU per ug of protein as determined by LAL method.
Formulation :
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution:
It is recommended to reconstitute the lyophilized recombinant Human Alkaline phosphatase (Intestinal type) /ALPI Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
Shipping:
The recombinant Human Alkaline phosphatase (Intestinal type) /ALPI Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage :
/strong>Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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