Name :
Siglec-3/CD33 Protein
Description :
Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state.They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs.
Species :
Human
Uniprotkb :
HEK293
Tag :
C-His-Avi
Synonyms :
p67, CD33 molecule, FLJ00391, Siglec3, Siglec-3, gp67, CD33
Construction :
Recombinant Human Siglec-3/CD33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Asp18-His259.[Accession |P20138-1]
Protein Purity :
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight :
The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
Endotoxin :
Less than 1EU per μg by the LAL method.
Formulatione :
Supplied as 0.22μm filtered solution in 20mM PB, 500mM NaCl (pH 8.0).
Reconstitution :
Stability & Storage :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background :
Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state.They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs.
References and Literature :
1. Walter RB, et al. Acute myeloid leukemia stem cells and CD33-targeted immunotherapy[J]. Blood, 2012, 119(26):6198-6208.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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