Cocos nucifera var. typica, commonly known as “gigante,” is a widely distributed coconut variety in tropical regions, particularly in Brazil, where it has been traditionally used in folk medicine for treating inflammatory conditions such as arthritis. Despite the documented biological activities of other C. nucifera varieties, the “gigante” variety remains understudied. This research evaluates the anti-inflammatory and antioxidant properties of an aqueous crude extract derived from the husk fiber of C. nucifera var. typica using in vivo and in vitro models. The extract was administered orally at doses of 10, 50, and 100 mg/kg in Swiss mice, with morphine (1 mg/kg) and acetylsalicylic acid (ASA, 100 mg/kg) serving as reference drugs. In the formalin-induced licking test, the extract significantly reduced the duration of licking during the second phase—associated with inflammatory pain—without affecting the first neurogenic phase, indicating specific anti-inflammatory action rather than general analgesia.Avelumab Protocol In the subcutaneous air pouch (SAP) model, carrageenan-induced inflammation led to increased leukocyte migration, protein extravasation, and tumor necrosis factor-alpha (TNF-α) levels. Pretreatment with the extract at 50 and 100 mg/kg significantly suppressed all three parameters, suggesting inhibition of inflammatory cell infiltration and mediator release. These findings imply that the extract interferes with key steps in the inflammatory cascade, possibly through modulation of cytokine production and vascular permeability.
The antioxidant potential of the extract was assessed using the DPPH photometric assay. Results demonstrated that the extract exhibited free radical scavenging activity comparable to standard antioxidants such as quercetin, rutin, and ascorbic acid, with an EC50 value indicating strong in vitro antioxidant capacity.2-Phenyl-1,3-propanediol Biochemical Assay Reagents This activity may be attributed to the presence of condensed tannins, specifically polymeric procyanidins, identified via HPLC/DAD analysis and confirmed by the vanillin-HCl assay, which detected procyanidin concentrations of 103.PMID:35006399 80 ± 10.5 mg/g of extract. Reactive oxygen species (ROS) are implicated in amplifying inflammatory responses by increasing endothelial permeability and promoting leukocyte transmigration; thus, the antioxidant effect of the extract may contribute directly to its anti-inflammatory profile. The dual functionality—combating oxidative stress and reducing inflammation—supports the traditional use of this plant in managing chronic inflammatory disorders.
In conclusion, the aqueous extract of C. nucifera var. typica demonstrates significant anti-inflammatory and antioxidant activities in experimental models. Its ability to inhibit inflammatory cell migration and TNF-α production, coupled with potent free radical scavenging capacity, underscores its therapeutic potential. These findings justify further investigation into the bioactive constituents responsible for these effects and open avenues for developing natural-based interventions for inflammatory diseases.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com