We have when compared gene delivery to experienced murine Purkinje neurons in vivo using AAV1 or lentiviral vectors with various promoter combinations and optimized for expression of two proteins in the exact same Purkinje neuron

Pursuing investigation of GFP expression, cells were immediately processed for Western blot evaluation, and membranes ended up probed with anti-GFP or antiFGF14 particular antibodies. Reliable with prior Western blot scientific…