July 2016

We therefore truncated the SLTxA gene soon after the codon expressing arginine 248 to produce a gene encoding SLTxA1 and fused this to the Kar2p sign peptide downstream of the galactose-inducible GAL1 promoter in a yeast expression vector derived from plasmid pRS316

The poisonous fraction of SLTxA1 demands specific residues of Hrd1p. A. Topological design of Hrd1p, based mostly on transmembrane phase prediction by the TOPCONS predictor , marked (white stuffed rings)…