Developed several advanced azobenzene derivatives.9-11 However, most of the photo-regulatory work can be achieved with the current commercial version. Since hybridization is a fundamental supramolecular property of DNA and most of the activity of DNA-based nanomachines is attributed to spontaneous hybridization, these machines can be easily converted to photo-responsive nanomachines just by adding azobenzenes into the sequence. Photo-regulated transcription and subsequent translation, i.e., photoregulated gene expression, is also possible with a photo-responsive promoter.
(a) Azobenzene amidite synthesized from D-threoninol for introducing an azobenzene unit into DNA. (b) Reversible photo-regulation of formation and dissociation of the duplex with photo-responsive DNA containing multiple azobenzene units.1194506-26-7 MedChemExpress (c) Reversible photo-switching of transcription by photoresponsive T7 promoter containing two azobenzene units. light clearly caused on-off photo-switching of RNA cleavage. PHOTO-REGULATION OF TRANSCRIPTION BY A PHOTORESPONSIVE PROMOTER Artificial control of biofunctions by external stimuli is one of the current hot topics in chemical biology or synthetic biology. Using azobenzene, a phototriggered gene expression system can be constructed. Isomerization of azobenzenes in the modified T7 promoter allows reversible photo-regulation of the transcription reaction by T7-RNA polymerase (Figure 1c).8 This regulation is based not on photoregulated hybridization but on a local structural change of the DNA duplex induced by photo-isomerization of azobenzenes between the trans and cis forms, allowing control of the DNA-protein interaction. We have developed an effective photoregulation design. In this design, the template strand remains intact and two azobenzenes are introduced into the nontemplate strand of the T7 promotor region. One azobenzene is inserted between -3 and
NEW PRODUCTS – DITHIOL SERINOL PHOSPHORAMIDITE AND 3′- DITHIOL SERINOL CPG
Gold nanoparticles (AuNPs) have become versatile tools for manufacturing biological sensors based on colorimetric, fluorescent, electrochemical, and other detection techniques. 1,2 AuNPs are of particular interest due to their ease of preparation and the diverse options for their functionalization.1,3 For example, aptamers in conjunction with AuNPs have been used for efficient target recognition by using the colorimetric change as the aptamer structure is modified when the target binds.2 For recent reviews on AuNPs, colloidal gold, and other nanomaterials, see Saha et al1 and Wang et al2. An essential component of the preparation of functionalized AuNPs is the method for attaching ligands to form activated nanoparticles. Due to the strong affinity of thiols and disulfides to gold surfaces and the ready availability of oligonucleotides functionalized with thiol groups at the 3′ or 5′ terminus, thiolmodified oligos have been used extensively for the preparation of oligonucleotide functionalized AuNPs.66225-78-3 site 4 However, Au-S bonds are susceptible to cleavage in the presence of other thiols or at elevated temperatures.PMID:30601618 5 Consequently, polythiols, such as a dithio-steroid or trebler-thiol-modified oligos, provide a higher level of stability over simple alkylthiol ligands.6,7 The trebler-thiol-modified oligos containing three alkylthiol ligands per molecule exhibited enhanced stability in AuNPs relative to dithiol-labelled oligos. The introduction of dithiol phosphoramidite (DTPA) offered a straightforward approach to.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com