Toductus and T.thermophilus could reflect either the specificity of their
Toductus and T.thermophilus may reflect either the specificity of their DNA uptake systems, or habitat specificity.It was found that the exceptionally frequent genomic rearrangements involving chromosomal and plasmid loci in Thermus genomes are moderated by internal mechanisms, which really most likely contribute for the adaptive evolution of these bacteria.Complete operons much more generally are transferred as entities, thus the rearrangements usuallyKumwenda et al.BMC Genomics , www.biomedcentral.comPage ofdo not disrupt syntenies of functionally associated genes.We did not find any correlation involving the rate of rearrangements and acquisitions of horizontally transferred genomic islands, but an growing trend was observed in rearrangement frequencies in intense thermophiles.Gene exchange by transformation had been identified to take place far more regularly between thermophilic T.scotoductus and Meiothermus instead of amongst the intense thermophiles.It might be explained either by the sharing of typical habitats with moderate thermophiles, or by the truth that naked DNA fragments degrade significantly more rapidly at incredibly higher temperature environments.More fileAdditional file Tree topologies for orthologous genes.
Background The sclerotium of Lignosus rhinocerotis (Cooke) Ryvarden or Tiger milk mushroom (Polyporales, Basidiomycota) is a important folk medicine for indigenous peoples in Southeast Asia.Regardless of the growing interest in this ethnobotanical mushroom, really small is known about the molecular and NSC-281668 Technical Information genetic basis of its medicinal and nutraceutical properties.Results The de novo assembled .Mb L.rhinocerotis genome encodes , putative genes with .of them possessing detectable sequence similarities to other people out there in public databases.Phylogenetic analysis revealed a close evolutionary partnership of L.rhinocerotis to Ganoderma lucidum, Dichomitus squalens, and Trametes versicolor within the core polyporoid clade.The L.rhinocerotis genome encodes a repertoire of enzymes engaged in carbohydrate and glycoconjugate metabolism, together with cytochrome Ps, putative bioactive proteins (lectins and fungal immunomodulatory proteins) and laccases.Other genes annotated include things like those encoding essential enzymes for secondary metabolite biosynthesis, which includes those from polyketide, nonribosomal peptide, and triterpenoid pathways.Among them, the L.rhinocerotis genome is specifically enriched with sesquiterpenoid biosynthesis genes.Conclusions The genome content of L.rhinocerotis offers insights in to the genetic basis of its reported medicinal properties at the same time as serving as a platform to further characterize putative bioactive proteins and secondary metabolite pathway enzymes and as a reference for comparative genomics of polyporoid fungi. Lignosus rhinocerotis, Genome, Phylogeny, Secondary metabolism, Carbohydrateactive enzymes, Cytochrome P superfamilyBackground Lignosus rhinocerotis (Cooke) Ryvarden, which belongs towards the family of Polyporaceae, is characterized by a centrally stipitate pilei arising from its distinct tuberlike sclerotium.This mushroom is broadly employed by natives of Southeast Asia as a basic health tonic for immune enhancement, or as a therapy regime for numerous ailments PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21324549/ such as cancer, asthma, and bronchitis.It is also utilized to treat discomfort triggered by fright, fever, coughing, vomiting, and cuts .The sclerotium may be the part of L.rhinocerotis with medicinal value.It truly is a compact Correspondence [email protected] Division of Molecular Medicine, Faculty of Medicine, Un.