Nd using the injection of MSCs medium. MSCs medium was found enriched with extracellular vesicles, hence leads to the concentrate on utilizing extracellular vesicles to treat neurological diseases, due to the proof that extracellular vesicles are able to penetrate the blood rain barrier. This project aims to create a solution with enriched extracellular vesicles and to evaluate its therapeutic efficacy in ischemic stroke. Techniques: MSCs, with similar passage number, have been derived from human-induced pluripotent stem cells-MSCs for the isolation of extracellular vesicles. The derived MSCs were then confirmed by the adherence to plastic, multipotent differentiation possible and surface antigen expressions. Three techniques (ultracentrifugation, ultrafiltration and polyethylene glycol) were employed to extract extracellular vesicles, which had been additional analysed by the expression of surface proteins, electron microscopy, ribosomal RNA detection and oxygen lucose deprivation (OGD) in vitro stroke model. Outcomes: Differentiated MSCs exhibited adherence to plastic, capability to differentiate into osteoblasts, adipocytes and chondroblasts, and 95 population expressed CD105, CD73 and CD90, and lack of CD45, CD34 and HLA class II. The isolated extracellular vesicles expressed CD9, CD63 and CD81, with all the size among 30 and 200 nm and contained RNA using a peak between 25 and 200 nucleotides. Goods from ultrafiltration have been identified to enhance cell viability in vitro stroke model most significantly. Summary/Conclusion: Extracellular vesicles were in a position to enhance the viability of neuronal cell (HT22) in oxygen lucose deprivation in vitro stroke model, indicating the potential use of extracellular vesicles injection as an option therapy for ischemic stroke. Funding: Innovation and Technologies Fund ITS-05317FX, the Government of your Hong Kong Particular Administrative Region.aimed to load EVs with Cre recombinase (Cre) as a model protein cargo and establish no matter whether functional delivery to cells could possibly be enhanced by using uptake-enhancing compounds. Methods: Expi293F cell line was utilized for isolating Cre loaded EVs by differential centrifugation just after transfecting releasing cells with constructs for protein expression. EVs were then analysed by nanoparticle tracking analysis, western blotting, RT-qPCR and cryo-electron microscopy such as detergent and nuclease digestion controls. Uptake of Cre loaded EVs was assessed employing modified Hek293T cells expressing a fluorescent reporter cassette consisting of LoxP GFP LoxP RFP. Final results: Endosomal escape enhancers chloroquine and Unc10217939 elevated TATcre functional delivery by 50 . CreFRB protein was loaded into EVs by rapaloginduced dimerisation to CD81FKBP. Cells treated with 20 /mL CreFRB loaded EVs showed functional Cre activity only inside the presence of 25 chloroquine or 2 unc10217939. Summary/Conclusion: CD11c/Integrin alpha X Proteins custom synthesis Passively loaded protein and mRNA was effectively delivered to recipient Hek293T fluorescent Cre reporter cells inside the presence of endosomal escape enhancing compounds. This BTNL9 Proteins medchemexpress locating shows that endosomal escape enhancing compounds could possess a location within the clinic to enhance delivery efficiency of nanoparticle-based therapies.PF11.14=OWP1.MSC exosome operates via a multifaceted mechanism of action in joint repair Shipin Zhanga, Yedan Wangb, Francis Keng Lin Wongc, Ming Wangb, Ruenn Chai Laid, James Hoi Po Huib, Sai Kiang Limd and Wei Seong Toha Faculty of Dentistry, National University of Singapore, Singapore, Sin.