Cle.supernatants of transfected HEK293T cells were harvested and subjected to a serial centrifugation protocol (300 g for 10 min, 2000 g for 10 min and 10,000 g for 30 min) to eliminate debris. Then, exosomes have been isolated from the cell culture medium by ultracentrifugation (150,000 for two h). Ferritin-SIRP and monomer SIRP proteins have been purified via an Ni-NTA chromatography stage. To the impartial comparison, we adjusted the same level of SIRP proteins of 2 nanocages in all experiments. Benefits: Exo-SIRP exceeds Ferritin-SIRP in all experiments, cell binding means, enhancing phagocytic perform of bone marrow derived macrophage, in vivo anti-tumour effect and tumour specific immune response. Exosome-SIRP exhibits better feasibility in contrast to ferritin-SIRP; five-folds higher inside the facet of cell binding capacity, 3 folds higher of phagocytosic ICAM-1/CD54 Proteins Biological Activity activity and 4 folds greater from the case of tumour growth inhibition. Summary/conclusion: We in contrast the efficacy of two nanoparticles and concluded that exosome has far more benefits in delivering membrane proteins for therapeutic objective. Our findings highlight the skill of exosomes to show native membrane proteins on their surface a substantial advantage of this delivery method and suggest that CD47 blockade by exosomemediated SIRP delivery is superior to that mediated by a Fc epsilon RI Proteins Formulation protein scaffold.LBS03.Comparison of exosomes and ferritin protein nanocages for the delivery of membrane protein theraqeutics Eunji Cho, Gi-Hoon Nam, Jiyoung Goo, Cherlhyun Jeong, Yoosoo Yang and In-San Kim Center for Theragnosis, Korea Institute of Science and Technologies, Seoul, Republic of KoreaLBS03.Cell-specific growth surface topography optimization for extracellular vesicle studies Colin L. Hiseya, Cherie Blenkironb and Larry Chamleyca University of Auckland, Grafton, New Zealand; bThe University of Auckland, Auckland, New Zealand; cThe University of AucklandIntroduction: Exosomes are little membrane vesicles secreted by most cell sorts that plays an important role in intercellular communication. Because of the characteristic of transferring their biomacromolecules, exosomes have possible as being a new alternative for delivering protein therapeutics. Right here, we investigate whether exosomes present critical positive aspects over other nanoparticles, particularly protein nanocage formulations, as being a delivery system for membrane protein therapeutics. We characterized membrane-scaffold ased exosomes and protein-scaffold ased ferritin nanocages, each harbouring SIRP (signal regulatory protein), an antagonist of CD47 on tumour cells. Approaches: For preparing exo-SIRP, HEK293T cells were transiently transfected with desirable plasmid DNA. Following a further incubation for 48 h, theIntroduction: While patient fluid samples give precious insight to the part of EVs in human health and fitness, their restricted provide and heterogeneous nature make them impractical for fundamental research. Conditioned media offers a consistent and limitless supply of EVs from a identified cell sort, but significant volumes are essential to make ample numbers of EVs. Also, minor is known about how aspects inside the cellular microenvironment, like surface topography, influence the EVs resulting from a lack of available biomimetic cell culture methods. We present a exclusive cell culture dish covered in microtrack patterns and show that this biomimicry influences the EVs developed by cancer cells. Methods: Microtrack patterns were fabricated using photolithography. Soft lithography was us.