D nanobeads, DNA molecules as well as other biomolecules. Funding: This study was supported by grants from your Minnesota Partnership for Biotechnology and Medical Genomics, MnDrive Investigation Initiative, NSF by means of the National Nanotechnology Coordinated Infrastructure (NNCI) plan, and inner project of KIST.PS04.A novel capture-and-release platform to isolate extracellular vesicle subpopulations reveals practical heterogeneity amongst EVs with diverse surface markers Olivier G. de Jonga, Mark Tielemansb, Raymond Schiffelersc, Pieter Vaderc and Sander A. A. Kooijmansca Division of Physiology, Anatomy and Genetics, University of Oxford, CD93 Proteins web Utrecht, Netherlands; bDepartment of Clinical Chemistry and Haematology, University Health care Center Utrecht, Utrecht, Netherlands; cLaboratory of Clinical Chemistry and Hematology, University Healthcare Center Utrecht, Utrecht, Netherlandsplatform to separate intact EVs depending on specific surface signatures and examine their properties. Approaches: EVs have been isolated from MDA-MB-231 cells employing size exclusion chromatography. EV subpopulations expressing certain surface markers had been captured on magnetic beads and released using a novel release protocol. Launched EVs have been characterized by western blotting, nanoparticle tracking examination (NTA) and transmission electron microscopy (TEM). Uptake of fluorescently labelled EV subpopulations by many cell styles was examined utilizing flow cytometry. Final results: Isolated MDA-MB-231 EVs showed typical EV properties, which include the presence of EV marker proteins, heterogeneous dimension distribution (mode dimension of 120 nm) by NTA and intact, “cup-shaped” morphology as visualized by TEM. When these EVs had been subjected to the capture-and-release platform, EV subpopulations with various properties had been obtained. Released subpopulations appeared intact as demonstrated by TEM, but differed in their dimension distribution. In addition, EV subpopulations showed different enrichment/depletion patterns of canonical EV CD48 Proteins Accession proteins as shown by western blot. Lastly, uptake of EVs by target cells differed concerning EV subpopulations and concerning target cell varieties. Summary/Conclusion: Within this get the job done we showcase a novel capture-and-release platform to separate intact EV subpopulations depending on their expression of precise surface markers. Using a smaller panel of antibodies against EV surface markers, we present differences among EV subpopulations when it comes to protein composition, dimension distribution and cellular uptake by target cells. We anticipate that this tool can assist to clarify relationships among the surface signature of EVs and their performance, and facilitate the enrichment of EVs with desirable qualities for therapeutic functions.PS04.Nanopillar and nanochannel fabrication by means of mixed lithography Sung-Wook Nama, Sun-Woong Leea and Moon-Chang Baekba School of Medicine, Kyungpook Nationwide University, Daegu, Republic of Korea; bSchool of medication, Kyungpook Nationwide University, Daegu, Republic of KoreaIntroduction: Extracellular vesicles (EVs) are heterogeneous when it comes to size and molecular composition, which may additionally reflect practical variations. By way of example, provided that the EV surface dictates interactions with their natural environment, EVs with distinct surface profiles could be taken up and processed by target cells in different techniques. Regrettably, tools to isolate and functionally evaluate EV subpopulations depending on their surface marker expression are at this time not accessible. Here, we describe a novel.