Rticipants. Outcomes: RNA sequencing identified a total quantity of 95 sEVs miRNA with differential expression amongst CC and NC, the majority of which (60/95) was in well accordance with tissue outcomes inside the Cancer Genome Atlas (TCGA) dataset. Among these miRNAs, we selected let-7b-3p, miR-139-3p, miR-145-3p, and miR-150-3p for further validation in an independent cohortconsisting of 134 participants (58 CC and 76 NC). Inside the validation cohort, the AUC of four individual miRNAs ranged from 0.680 to 0.792. A logistic model combining two miRNA (i.e. let-7b-3p and miR-145-3p) accomplished an AUC of 0.901. Adding the 3rd miRNA (miR-139-3p) into this model can further enhance the AUC to 0.927. Side by side comparison revealed that sEVs miRNA profile outperformed cell-free plasma miRNA within the diagnosis of early CC. Summary/Conclusion: Circulating sEVs possess a distinct miRNA profile in CC individuals, and sEVs derived miRNA may be utilised as a promising biomarker to detect CC at an early stage. Funding: This operate was supported by grants in the National Natural Science Foundation of China (81702314).JOURNAL OF EXTRACELLULAR VESICLESSymposium Session 20: EV Therapeutics II Chairs: Minh Le; Lucia Languino Place: Level B1, Hall B 16:308:OF20.Nano-Ghosts: mesenchymal stem cells derived nanoparticles as a novel method for cartilage regeneration. Domenico D’Atria, Joao Garciab, Laura Creemersc and Marcelle MachlufdaTechnion Israel Institute of Technologies, Haifa, Israel; bUMC Utrecht, Utrecht, Netherlands; cDept Orthopaedics, University Healthcare Centre Utrecht, Utrecht, Netherlands; dTechnion Israel Institute of Technology, Haifa, Israelstandalone biological or as a carrier for the targeted delivery of therapeutics, which include anti-inflammatory agents and development elements. Ongoing in vivo research are focusing on confirming the NGs’ targeting and anti-inflammatory capacity. Funding: This project has received funding in the European Union’s CD15 Proteins custom synthesis Horizon 2020 research and innovation programme below Marie Sklodowska-Curie grant agreement NoIntroduction: Osteoarthritis could be the most common inflammatory illness on the joints which is characterized by cartilage degeneration and bony overgrowth. Mesenchymal stem cells (MSCs) play an vital function in inflammation, due to their aptitude to household to inflamed tissues and modulate the CD14 Proteins MedChemExpress process. We created a brand new type of particles termed Nano-Ghosts (NGs), derived in the cytoplasmic membrane of the MSCs. Retaining MSCs’ surface properties, NGs are anticipated to target inflamed tissue and modulate inflammation. In this study, we demonstrate NGs’ ability to target human articular chondrocytes (hACs) and cartilage explants even though reducing inflammation. Approaches: Targeting was evaluated by flow cytometry and confocal microscopy. NGs’ anti-inflammatory properties had been studied in vitro on TNF-stimulated and non-stimulated hACs and, ex vivo, on cartilage explants. qPCR and ELISA of numerous markers assessed anti-inflammatory impact. Smooth muscle cell (SMC)NGs have been used as a non-MSC manage. Outcomes: Flow cytometry showed that NGs can target hACs’ two instances extra efficiently in comparison to SMC-NGs. Moreover, NGs showed 4 occasions larger targeting to TNF-stimulated hACs. Targeting was confirmed by confocal microscopy and imaging flow cytometry which showed that NGs bound the membrane and had been taken up by the cells. Similar outcomes were achieved in human explants where the particles showed four instances higher binding to TNF-stimulated explants. To test the anti-inf.