Icles. We’ve got lately enhanced the contrast and spatial resolution of SPIRI by pupil function engineering and computational imaging. Techniques: In SPIRI, the interference of light reflected in the sensor surface is modified by the presence of particles making a distinct signal that reveals the size with the particle that is not otherwise visible under a standard microscope. Applying this instrument platform, we’ve demonstrated label-free identification and visualization of numerous viruses in multiplexed format in complex samples inside a disposable cartridge. Not too long ago, our technology was applied to detection of exosomes and commercialized by Nanoview Biosciences for quantified measurement of exosomes on dry sensor chips. We are currently focusing onISEV2019 ABSTRACT BOOKvarious in-liquid detection also as additional improvement with the method utilizing pupil function engineering. Benefits: By acquiring several pictures having a partitioned pupil (resulting in structured illumination) and computational imaging, we’ve demonstrated significant improvement in visibility of low-index nanoparticles in liquid. Furthermore, spatial resolution has been improved beyond the diffraction limit approaching 100 nm in the visible microscopy. We’ve got created compact and cheap sensor chips and microfluidic cartridges permitting for study of biological particles (exosomes and also other extracellular vesicles) straight in the bodily fluids without TNF-R2/CD120b Proteins Synonyms labels. Summary/Conclusion: In summary, we have demonstrated improved visibility of exosomes in SPIRI employing pupil function engineering. Funding: EU-INDEXuse of many recognition events in mixture with signal N-Cadherin/CD325 Proteins Synonyms amplification enables detection of exosomes with high specificity and sensitivity. Summary/Conclusion: Here, we talk about the application of proximity assays for sensitive detection of exosomes in body fluids, to visualize the uptake of exosomes by cells, and the possible of such approach to become utilized to superior fully grasp the biology from the exosomes and to determine exosomes as illness biomarkers.OF22.A 96 well plate format lipid quantification assay with enhanced sensitivity for standardization of experiments with extracellular vesicles Tamas Visnovitza, Xabier Osteikoetxeab, Barbara W. S arc, Judith Mihalyd, P er Lrincze, Krisztina V. Vukmana, Eszter nes T ha, Anna Koncza, Inna Sz sf, Robert Horv hf, Zoltan Vargag and Edit I Buz c Semmelweis University, Dept. of Genetics, Cell- and Immunobiology, Budapest, Hungary; bAstraZeneca, Macclesfield, UK; cSemmelweis University, Budapest, Hungary; dRCNS HAS, Budapest, Hungary; e Department of Anatomy, Cell and Developmental Biology, E v Lor d University, Budapest, Hungary; fNanobiosensorics Laboratory MTA-EKMFA, Budapest, Hungary; gResearch Centre for All-natural Sciences, Hungarian Academy of Sciences, Budapest, HungaryaOF22.Proximity assays for detection and characterization of exosomes Masood Kamali-Moghaddam, Ehsan Manouchehri, Alireza Azimi, Qiujin Shen, Radiosa Gallini and Claudia Fredolini Uppsala University, Uppsala, SwedenIntroduction: Exosomes acquire an elevated consideration in basic biology as well as in medicine. They’re shown to be involved in a lot of biological processes, and are proven to hold excellent potentials as diagnostic and therapeutic tools. Even so, there’s an unmet will need for new and improved technologies for quantitative and qualitative characterization of exosomes to meet challenges related to these vesicles, including low concentrations in physique f.