Odies in relevant in vitro models. Coupling immune-isolation to pre-analytical protocols for urine processing and sample excellent testing enables αLβ2 Purity & Documentation detection of a panel of endometrial genes in urine-recovered EVs. Summary/Conclusion: All round, the study supplies a tool for non-invasive monitoring of your functional status on the endometrium, supporting biomedical niches including assisted fertilization and diagnosis of endometriosis. Funding: ENDEvor POR Area Tuscany (identification of the project) and Exosomics R D ProgrammePF09.Unveiling autologous blood doping: comparative analysis of distinct purification approaches for urinary extracellular vesicles pioneering miRNA biomarker investigation Veronika Mussacka, Georg Wittmannb and Michael Pfafflc TUM College of Life Sciences Weihenstephan, Division of Animal Physiology and Immunology, Freising, Germany, Freising, Germany; bDepartment for Transfusion Medicine, Cell therapeutics and Haemostaseology, University Hospital LMU, Munich, Germany, M chen, Germany; cAnimal Physiology and Immunology, College of Life Sciences Weihenstephan, Technical University of Munich, Freising, Germany, Freising, Germanyafive different isolation techniques were evaluated: ultracentrifugation, membrane affinity, spin column chromatography, immunoaffinity and precipitation. Just after EV characterization by nanoparticle tracking evaluation, western blotting, and transmission electron microscopy, total RNA was isolated and also a library for small RNA sequencing was ready. The resultant prosperous tactic was then applied to all the collected samples which had been equally analysed with regards to their EV distribution and miRNA content material. Outcomes: The comparative evaluation disclosed massive discrepancies with respect to EV yield, population, and purity, also as RNA yield and detected miRNAs. By applying the ideal performing method, which was determined by immunoaffinity, substantially greater amounts of urinary EVs and several substantially differentially regulated miRNAs have been observed immediately after ABD. Summary/Conclusion: Urinary EVs and their miRNA profile hold indeed promising attempts for the clear separation of ABD and non-doped athletes. Additionally, the integrated complicated comparative methodological analysis PARP3 custom synthesis contributes enormously to future standardization and comparability of urinary EV research. Funding: The current project has been financially supported by the WADA.PF09.Extracellular vesicles as graft biomarkers to address lung transplantation outcome Mario Barilania, Ilaria Righib, Giuseppe Buonoc, Lorenzo Rossod, Mario Nosottie and Lorenza Lazzarica Unit of Regenerative Medicine Cell Factory, Department of Transfusional Medicine and Hematology, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milano (MI), Italy; Universitdegli Studi di Milano, Milan, Italy; bThoracic Surgery and Lung Transplant Unit, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milano (MI), Italy, Milano, Italy; cUnit of Regenerative Medicine Cell Factory, Division of Transfusional Medicine and Hematology, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milano (MI), Italy, Milano, Italy; dUniversitdegli Studi di Milano, Milano (MI), Italy, Milano, ItalyIntroduction: Autologous blood doping (ABD) increases the oxygen capacity by means of re-infusion of a person’s autologous red blood cells. It really is therefore utilized by endurance athletes using a higher level of unreported cases, in particular because dependable approaches for unequivocal detection are still lacking. To su.