Cargos which include proteins and nucleic acids. To accurately and particularly quantify tumourderived EVs from complex biofluids like human plasma is potentially substantial for precise diagnosis. Numerous SIRT6 site strategies for EVs quantification have already been developed in the past decade, such as nanoparticles tracking evaluation, total internal reflection fluorescence microscopy, flow cytometry and enzyme-linked immunosorbent assays (ELISA). On the other hand, bulky and pricey instruments are necessary for these approaches. Therefore, this study provides a basic and low-cost strategy to quantify circulating EVs from human plasma by using the ELISA method and a fluorescent microscope on a membrane-based integrated microfluidic platform. Methods: Within this study, a membrane-based integrated microfluidic platform was used for EVs collection,ISEV2019 ABSTRACT BOOKenrichment and fluorescent detection approach. A tracketched membrane filter using a pore size of 0.03 m that could enrich EVs and deplete small molecules throughout washing methods was packaged within a polydimethylsiloxanebased microfluidic platform. Following EVs enriching, an on-chip ELISA assay was performed involving the following methods including (1) anti-CD63 antibody (EPR5702) incubation, (two) horseradish peroxidase (HRP) conjugated anti-rabbit antibody incubation, and (3) tetramethylrhodamine-labelled tyramide incubation. It is actually worth noting that tyramide molecules could possibly be accumulated on the surface of EVs to amplify the fluorescent signal and observed under a fluorescent microscope. With this strategy, absolute quantification of EVs with high specificity could be accomplished. Results: The experimental outcomes showed that CD63positive circulating EVs in human plasma could be individually observed under a fluorescent microscope. By utilizing imaging application (ImageJ) to carry out image evaluation, the total variety of EVs might be quantified such that the concentration of EVs in plasma may be measured. Summary/Conclusion: The developed system could be utilized to quantify EVs with high specificity and could be broadly utilized in most general laboratory for precise diagnosis of circulating EVs from human plasma. Funding: Ministry of Science and Technologies of Taiwan (MOST 106221-E-00701, MOST 1072221-E-00713-MY3)volume and reagent consumption. To solve many technical troubles involving the generation of electrolysis gas around the electrodes, many of the micro-FFE devices reported in the past had been fabricated employing elaborate micromachining approach on silicon or glass substrates. Nevertheless, high-cost micromachining processes have been necessary, and these have been not suitable for mass production. Outcomes: Determined by these backgrounds, we recently created a polymer-based easy-to-fabricate microFFE device and overcame the difficulties talked about above. In this presentation, we will introduce the application of this device to EV separations in this presentation. Electrophoretic separation of Sk-Br-3 derived exosomes expressed with HER2 antigen were demonstrated with and without the need of the combination use with the anti-HER2 antibody for molecular particular separation. Summary/Conclusion: The present process will probably be among the promising candidates for separating favourable kinds of EVs from heterogeneous samples. Funding: Center of Innovation Program (COI STREAM) from Japan Science and Technologies Agency (JST)PT09.Size distribution of extracellular vesicles by microfluidic resistive pulse sensing and small-angle neutron NK3 Biological Activity scattering Zoltan Vargaa, Bence Feherb, Diana Ki.