Enitor neurons) and collagen IV (angiogenesis) of ipsilateral. EVs groups showed substantially increased co-expression of ki-67 and DCX inside the subventricular zone. Enhanced expression of collagen IV in the ischemic boarder zone was more located in the EV groups than within the control group. Diffusion tensor imaging was used to examine the regeneration of nerve fibre bundles, and nerve fibre bundles have been also enhanced within the EV groups. Summary/Conclusion: Our study shows that WJ-MSC derived EVs from 3D culture program promotes the neurogenesis, angiogenesis and recovery of nerve fibre bundles in rat stroke models. These benefits suggest that an efficient MSC-derived EV may be applied ideally for the treatment of stroke. Funding: This study was supported by a grant from the Korean Healthcare Technology R D Project, Ministry of Health Welfare (HI17C1256) and Standard Science Study Program, the Ministry of Science, ICT and Future Organizing (2018M3A9H1023675).PF11.Exosomes from human urine-derived stem cells promote neurogenesis through histone deacetylase6 regulation in ischemic stroke Xiaozheng Linga, Zhang Guoweia, Qingwei Zhua, Hu Guowena, Yang Wangb and Zhifeng Dengaa Shanghai Jiao Tong University Affliated Sixth People’s Hospital, Shanghai, China (People’s Republic); bShanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, China (People’s Republic)PF11.The therapeutic prospective of MSC-derived extracellular vesicles for stroke Ji Hee Sunga, Eun Kyoung Shinb, Jeong Pyo Sonc, Yeon Hee Choa, Dong Hee Kimd, Mi Jeong Ohb, Eun Hee Kimb, Jae Min Chae, Oh Young Bangfa Samsung healthcare center, seoul, Republic of Korea; bSamsung health-related center, Seoul, Republic of Korea; cSungkyunkwan University, Seoul, Republic of Korea; d Sungkyunkwan University, seoul, Republic of Korea; eDepartment of Mechatronics, College of Engineering, Incheon National University, Incheon, Republic of Korea; fSamsung medical center, Seoul, Republic of KoreaIntroduction: Human urine-derived stem cells (USCs) are receiving considerably far more consideration in tissue regeneration and injury repair. Exosomes derived from USCsISEV2019 ABSTRACT BOOK(USCs-Exo) have recently been recommended to mediate the restorative effects of USCs. Nonetheless, no matter if USCs-Exo play a protective function in stroke remains unknown. Approaches: Therapeutic impact of USCs-Exo in vivo was evaluated by cerebral infarction and neurological behaviour. The proliferation and differentiation of neural stem cells (NSCs) was determined by double immunofluorescent staining. The MMP-2 Compound viability, apoptosis, proliferation, and differentiation of NSCs subjected to oxygen glucose deprivation/reoxygenation (OGD/R) were assessed, respectively. The protein expression and activity of HDAC6, plus the expression levels of miRNAs both in vivo and in vitro had been assessed to detect the achievable mechanism. Outcomes: We discovered that intravenous injection of USCsExo reduced brain infarct volume and PLK4 supplier improved functional recovery by enhancing the proliferation and differentiation of NSCs in ischemic rats. The in vitro results recommended that USCs-Exo improve viability, cut down apoptosis, and promote the proliferation and neuronal differentiation of NSCs right after OGD/R. We further found that miR-206 contained in USCs-Exo is connected using the therapeutic efficacy for neurogenesis by way of the inhibition of histone deacetylase6 (HDAC6). Summary/Conclusion: Our study demonstrates that USCs-Exo can boost neurological function recovery by promoting neurogenesis, wh.