Recommended that the HpaBC complicated has in vivo activity towards p-CA, and Af, with high catalytic efficiency. Our Our final results showed activity towards p-CA, N, N, and Af, with high catalytic efficiency. final results showed that that those substrates (except anthocyanin) could possibly be converted for the corresponding orthothose substrates (except anthocyanin) could possibly be converted towards the corresponding ortho-prodproducts the optimaloptimal circumstances. Similarly, the conversion of those by P2 3ucts below beneath the situations. Similarly, the conversion of those substrates substrates by P2 3-carrying also drastically larger than higher than that of P2-carrying strains. carrying strains was strains was also considerably that of P2-carrying strains. The converThe conversion to E was 58 , PKC Formulation followed by that for Af by that three.14), p-CA (32.93 two.01), sion price from N rate from N to E was 58 , followed (35.two for Af (35.2 3.14), p-CA (32.93 2.01), DHK (23.74 1.75) and K (23.84 0.88), when substrate PEL was not PEL DHK (23.74 1.75) and K (23.84 0.88), when activity on the activity on the substratedewas not detected (Table 2). These final results α5β1 Storage & Stability supplied proof that the HpaBC complicated had tected (Table two). These outcomes supplied proof that the HpaBC complex had in vivo in vivo activity towards monohydroxylated flavanol and flavonol. activity towards monohydroxylated flavanol and flavonol.Molecules 2021, 26, 2919 Molecules 2021, 26, x FOR PEER REVIEW9 of 13 9 ofFigure six. HPLC evaluation of your enzymatic merchandise with the HpaBC complicated, when feeding with different substrates. HPLC Figure six. HPLC analysis of the enzymatic merchandise on the HpaBC complicated, when feeding with unique substrates. HPLC chromatogram chromatogram (left) and normal compound (ideal) analyses of the enzymatic reaction. N, E,E, K, Q, DHK, DHQ, and Af common compound (suitable) analyses in the enzymatic reaction. N, K, Q, DHK, DHQ, C C and had been monitored at at nm, and p-CA and CA have been monitored at 340 340 The substrates and and corresponding products Af had been monitored280280 nm, and p-CA and CA have been monitored at nm. nm. The substrates corresponding goods have been had been detected by HPLCLC-MS. The ortho-hydroxylation activities of (a): p-CAp-CA to (b): N to N to E; (c): Af to C;K to Q; detected by HPLC and and 2021, 26, x FOR PEER Overview Molecules 2021, 26,Molecules LC-MS. The ortho-hydroxylation activities of (a): to CA; CA; (b): E; (c): Af to C; (d): (d): K x FOR PEER Critique to Q;(e): DHKDHK to DHQ. Final substrate concentration of 80 mg-1, three. = three. L and and (e): to DHQ. Final substrate concentration of 80 mg -1 , n = n10 ofTable two. The yield and conversion price of ortho-hydroxylated flavonoids for the HpaBC complicated Table 2. The yield and2. The yield and conversion price of ortho-hydroxylated flavonoidscomplex when feeding with difTable conversion rate of ortho-hydroxylated flavonoids for the HpaBC for the HpaBC complex when feedin when feeding with various substrates. The horizontal lines within the table indicate that no activities has ferent substrates. The horizontal lines horizontal lines inside the table indicate that no activities has Information are shownData are sho ferent substrates. The in the table indicate that no activities has been detected. been detected. as the been implies s.d.s (n suggests s.d.s (n =Data are shown because the means s.d.s (n = 3). = 3). detected. 3).SubstratesSubstrates SubstratesProducts ProductsYield Yield Conversion Rate ( ) Conver L Conversion Merchandise (mg )(mg-1) Yield (mg-1) Rate ( ) L-2.