Nutrient limited situations (DDN, AN, DDP, and AP). = probability value (important level); 0.05.and DDP (101.6833 mg/g), even though the AN led to declined total protein content material in the cells (76.48 mg/g) (Figure two). The interaction of protein with nitrate and phosphate concentration was substantial in DDN, AP, and DDP treated cells ( sirtuininhibitor 0.05) except for the AP treated cells ( sirtuininhibitor 0.05) (Figure two). It has been observed that, in Gracilaria and Ulva, the total protein content was elevated significantly right after 10 d of ammonium enrichment [9]. Carbohydrate and protein are among by far the most crucial elements involved in metabolism for they provide energy for development and cellular differentiation. Seasonal variation of total carbohydrate and protein content material with the marine macroalgae Enteromorpha intestinalis, Ulva lactuca, and Catenella repens from coastal West Bengal was studied by Banerjee et al. [44]. It has been previously reported that carbohydrate accumulation is inversely proportional towards the lipid production, since the lipid precursor glycerol-3phosphate is developed by glucose metabolism [45, 46]. three.four. Changes of Total Lipid. Twofold increase in total lipid content (193.CD3 epsilon Protein medchemexpress 03 mg/g) was discovered in AN treated biomass of Rhizoclonium (Figure 2). In addition, the AP resulted in lipidInternational Journal of MicrobiologyTable 1: Showing fatty acid compositions ( ) of each handle and treated cells (DDN, AN, DDP, and AP) of R. africanum at log-exponential phase (14-day-old culture). Fatty acids 12:0 14:0 15:0 16:0 16:1 16:2 16:three 17:0 18:0 18:1 18:2 18:three (GLA) 18:3 (ALA) 20:0 20:1 20:2 20:3 20:4 20:five 22:0 24:0 Fatty acid compositions ( ) -NO3 (AN) (14 days) — four 1.1 32.6 13.1 1 0.2 — 1.3 29.two 4.1 0.9 — 0.six 1.7 0.9 two — — — 12.1 -PO4 (AP) (14 days) 0.six five.two two 40.9 12.2 1.five 0.eight — 1.3 23.9 5.1 1.2 — — 1.5 — — — — — six.Manage (14 days) three.4 6.five two.eight 30.2 9.four — — — 1.four 20 five.3 — 7.four — — — 2.4 — — — 11.Siglec-9 Protein Formulation +NO3 (DDN) (14 days) 1.1 four.9 1.two 34.9 11.two 0.8 0.9 — 1.3 21.2 five.3 1.1 — — 1.3 — 1.two — — — 11.+PO4 (DDP) (14 days) 0.5 6.6 two.4 29.PMID:24324376 9 11.two 1.six five.two 0.2 0.6 20.two 10.7 1.7 — — 0.four 1.4 3.6 — — 0.two three.1.6 1.four 1.two 1 0.eight 0.6 0.four 0.two 0 Manage DDN AN DDP Abiotic anxiety parameters APFigure 4: Bar graphs showing lipid peroxidase activity (MDA content material) of each control and treated biomass (DDN, AN, DDP, and AP) of R. africanum. = probability value (significant level); 0.05.[20, 41, 58, 59]. The SFAs had been located as C12:0 , C14:0 , C16:0 , C16:two , C17:0 , C18:0 , C22:0 , and C24:0 . Amongst these, C16:0 created up the highest proportion beneath each manage (30.two ) and strain circumstances (40.9 ). The AN remedy resulted in enhanced biosynthesis of MUFAs soon after a 14 d exposure, that’s, C16:1 , C18:1 , and C20:1 (13.1, 29.two, and 1.7 inside the treated cells compared to 9.4, 20, and 0 within the control, resp.) (Table 1). The fatty acid profile of Ulva rigida showed dominance of both saturated and unsaturated fatty acids [10]. The fatty acidprofile of macrophytic algae Egregia menziesii (Phaeophyta), Chondracanthus canaliculatus (Rhodophyta), and Ulva lobata (Chlorophyta) showed dominant fatty acids of C16:0 and other fatty acid classes had been identified as C14:0 , C18:1 , C20:4 , and C20:five [51]. They also reported that the C22 PUFA had been exceptional to U. lobata. The fatty acid evaluation of Ulva reticulata showed dominance of C16:0 and C14:0 (50.76 and 11.77 ) below control circumstances [15]. The present investigation group reported fatty acid profiles of two.