Product Name :
Androgen Receptor

Description :
| Clone F39.4.1 | Isotype IgG1 | Product Type Monoclonal Antibody | Units 1 ml | Host Mouse | Species Reactivity Human | Application Immunohistochemistry (frozen) Immunohistochemistry (paraffin)

Background :
This monoclonal antibody is specifically designed to recognize a unique immunogenic N-terminal transactivation domain of the androgen receptor that has a low degree of homology with other steroid receptors. This antibody binds to synthetic peptide SP61 (amino acids 301-320) of human androgen receptor as assessed by ELISA and recognizes a 100 kD human androgen receptor protein in Western blot analysis. In addition, this antibody binds to native human androgen receptor in immunoprecipitation, sucrose gradient centrifugation, and immunohistochemistry. This antibody does not cross-react with human estrogen, progesterone or glucocorticoid receptor. Immunogen: Synthetic peptide

Source :
The antibody to androgen receptors binds to these proteins in nuclei of typical normal tissues. Over-expression may be observed in tumours like prostate carcinoma and osteosarcoma. For information transmission to the target cells male steroid hormones like Testosterone and DHT require a specific receptor. This specific receptor, called androgen receptor, is found in numerous human tissues like skin, mucous membranes, seminal vesicles, prostate gland and testis. Androgen receptors will also be found in human tumours like prostate carcinoma, osteosarcoma and others. In prostate carcinoma the clinical relevance has been proven by castration and androgen deprivation by Gn-RH-agonist and -antagonist treatment. In many cases androgen deprivation slows down tumour growth in androgen-dependent prostate carcinoma. Human androgen receptor, 110 kDa protein in lysate of LNCP cell line.. <

Product :
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002. Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002. Secondary Reagents: We recommend the use of BIOLOGO’s Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Specificity :
Species Reactivity: Human

Applications :
IHC(C, P) Incubation Time: 60 min at RT Working Concentration: (liquid conc.) 1:5 – 1:20 Pre-Treatment: Heat pre-treatment of formalin-fixed tissue with Unmasking Fluid C or G (Art. No. DE000 and DE007) Positive Control: Testis

Storage :

Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References :
1. Veldscholte J., Berrevoets C.A., Zegers N.D., van der Kwast T.H., Grootegoed J.A., and Mulder E. (1992) Hormone -induced dissociation of the androgen receptor-heat-shock-protein complex: use of a new monoclonal antibody to distinguish transformed from nontransformed receptors. Biochem. 31(32);7422-7430. 2. Iwamura M., Abrahamsson P.A., Benning C.M., Cockett A.T., di Sant’Agnese P.A. (1994) Androgen receptor immunostaining and its tissue distribution in formalin-fixed paraffin-embedded sections after microwave treatment. J. Histochem. Cytochem. 42(6); 783-788. 3. Shikata N., Kurokawa I., Andachi H., Tsubura A. Expression of androgen receptors in skin appendage tumors: an immunohistochemical study. J. Cutan. Pathol. 22(2); 149-153. 4. Magi-Galluzzi C., Montironi R., Prete E., Kwan P.W., and Delellis R.A. (1996) Effect of microwave oven heating times on androgen receptor antigen retrieval from paraffin-embedded prostatic adenocarcinoma. Anticancer Res. 16(5A9; 2931-2936.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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