Product Name :
Chicken anti Green Fluorescent Protein (GFP) [Neisseria gonorrhoeae]

Description :
| Product Type Primary antibodies | Units 10 mg | Host Chicken | Application ELISA Western Blotting

Background :
Chickens were immunized with recombinant full-length bacterial gonococci Green Fluorescent Protein (GFP) [Neisseria gonorrhoeae] expressed in E. coli cells. After multiple immunizations in Freund’s adjuvant, eggs were collected and the IgY purified.

Source :

Product :
Product Form: Egg yolk-derived purified IgY Formulation: Phosphate buffered saline‚ pH 7.2 with 0.075% sodium azide Purification Method: Egg yolk-derived purified IgY (non affinity purified) Concentration: See vial for concentration.

Specificity :

Applications :
In a Western blot, the anti-GFP used at between 1:3000 and 1:5000 dilution could detect 10 ng of GFP in an A549 cell lysate (see image). Optimal conjugate working dilutions should be determined for your particular assay conditions.

Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles. Shipping Conditions: Ship at ambient temperature.

Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References :

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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