Product Name :
Mouse anti Green Fluorescent Protein (GFP)

Description :
| Clone F56-BA1.2.3 | Isotype IgG | Product Type Monoclonal Antibody | Units 100 µg | Host Mouse | Species Reactivity Jellyfish | Application Western Blotting

Background :
Hybridoma produced by the fusion of splenocytes of mice immunized with full-length green fluorescent protein and mouse myeloma cells.

Source :
Green Fluorescent Protein (GFP) from Jellyfish (Aequorea Victoria) is a 27kDa protein that provides strong visible fluorescence. GFP can be used as a reporter of gene expression, a tracer of cell lineage, and a fusion tag to monitor protein localization from living cells. It can also be used to access protein-protein interactions in yeast two hybrid systems and measure distances between proteins in fluorescence energy transfer (FRET). <

Product :
Product Form: Unconjugated Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide Purification Method: Protein A/G Chromatography Concentration: See vial for concentration

Specificity :

Applications :
Antibody can be used for Western blotting (1-10 µg/ml). Optimal concentration should be evaluated by serial dilutions. Functional Analysis: Western Blotting Positive Control: Purified Green Fluorescent Protein

Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References :
1. Prasher,D.C ‘Primary structure of the Aequorea victoria green-fluorescentprotein.’ Gene 111 (2), 229-233 (1992) 2. Inouye,S. and Tsuji,F.I. ‘Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein’ FEBS Lett. 341 (2-3), 277-280 (1994) 3. Cody, ‘Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein’ Biochemistry 32 (5), 1212-1218 (1993

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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