Product Name :
Mouse anti Human Calpain I, II (small subunit)
Description :
| Clone 28F3 | Isotype IgG1 | Product Type Monoclonal Antibody | Units 100 µg | Host Mouse | Species Reactivity Bovine Human | Application Enzyme Immunoassay Immunoprecipitation Western Blotting
Background :
Hybridoma produced by the fusion of splenocytes from mice immunized with human calpain with mouse myeloma.
Source :
The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and µ-calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar (mM) levels of calcium, µ-calpain is active at micromolar (µM) concentrations of Ca ++. In addition to the ubiquitously expressed m- and µcalpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux. Synonyms: Mouse anti calpain I, II small subunit, p30 <
Product :
Provided as a concentration of 1 mg/ml in 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50% glycerol, 3mM sodium azide. Product Form: Unconjugated Purification Method: Protein A/G Chromatography Concentration: See vial for concentration
Specificity :
Applications :
Use at 0.5-1 ug/ml for ELISA and western blot, 1-2ug/ml for immunoprecipitation. Functional Analysis: Western Blotting
Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
1. Adamec, E., et al. ‘Calpain I activation in rat hippocampal neurons in culture is NMDA receptor selective and not essential for excitotoxic cell death.’ Brain Res. Mol. Brain Res. 1998, 54, 35-48 2. Guttmann, R.P. et al. ‘Oxidation inhibits substrate proteolysis by calpain I but not autolysis.’ J. Biol. Chem. 1997, 272, 2005-2012
Related websites: https://www.medchemexpress.com/antibodies.html
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