Product Name :
Mouse anti Human Caspase-9
Description :
| Clone LAAP6 96 2-22 | Isotype IgG1, kappa | Product Type Monoclonal Antibody | Units 100 µg | Host Mouse | Species Reactivity Human | Application Western Blotting
Background :
Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human capase-9 protein and mouse myeloma cells.
Source :
Caspases are key effectors of programmed cell death. They are synthesized as inactive proenzymes which are activated by cleavage at a specific aspartate residue to form two subunits. These subunits are normally linked together by a linker which may be involved in the regulation of the different caspases. Caspase-9 is a member of the CED-3 family and bear high similarity to caspase-3. Procaspase-9 can be activated by either caspase-3 or granzyme B, although they cleave the proenzyme to different size subunits. Cleavage by granzyme B produces an active enzyme which is capable of cleaving PARP. Also, the ability of caspase-3 to activate caspase-9 seems to suggest that caspase-9 is further downstream of caspase-3 and may be involved in later changes in cells observed undergoing apoptosis. Synonyms: Cysteine-requiring Aspartate Protease-9; iCE-like apoptotic protease 6;Apoptotic protease Mch-6; Apoptotic protease-activating factor 3 <
Product :
Product Form: Unconjugated Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide Purification Method: Protein A/G Chromatography Concentration: See vial for concentration
Specificity :
Applications :
Detects proenzyme form of human Caspase-9 by Western blot analysis as well as one of the cleavage products after treatment with thapsigargin. Optimal concentration should be evaluated by serial dilutions. Functional Analysis: Western Blotting Positive Control: MCF-7 cell lines
Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
1. Cohen, G.M., et al. Caspases: the executioners of apoptosis. Biochem. J. 1997, 326, 1-16 2. Stennicke, H.R., et al. Caspase-9 can be activated without proteolytic processing. J. Biol. Chem. 1999, 274, 8359-8362 3. Kuida, K. Caspase-9. Int. J. Biochem. Cell Biol. 2000, 32, 121-124
Related websites: https://www.medchemexpress.com/antibodies.html
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