Product Name :
Mouse anti Human CD18, conjugated with Biotin
Description :
| Clone 3EB | Isotype IgG1 | Product Type Single-Color Reagent | Units 100 Tests | Host Mouse | Species Reactivity Human | Application Flow Cytometry
Background :
Source :
LFA-1 beta is a leukocyte adhesion receptor to (ICAM’s) intercellular adhesion molecules. LFA-1 beta is on virtually all peripheral blood lymphocytes, 95,000 M.W. surface antigen. Synonyms: CD18* Biotin <
Product :
Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein Product Form: Biotin Purification Method: Protein A/G Chromatography Concentration: Titered for flow cytometry
Specificity :
Applications :
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2° to 8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20°C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. Functional Analysis: Flow Cytometry Staining
Storage :
Product should be stored at 4-8°C. DO NOT FREEZE Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
1. CD11c/CD18 on neutrophils recognizes a domain at the N terminus of the alpha chain of fibrinogen. Loike, J.D., Sodeik, B., Cao, L., Leucona, S., Weitz, J.I., Detmers, P.A., Wright, S.D., Silverstein, S.C.. Proc. Natl. Acad. Sci. USA, 1991 Feb; 88(3):1044-8. 2. T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1. Dustin, M.L. and Springer, T.A.. Nature. 1989:341:619. 3. Coupling of the adhesive receptor CD11/CD18 to functional enhancement of effector macrophage tissue factor response. Fan, S.T. and Edgington, T.S.. J. Clin. Invest. 1991 Ja:87(1):50-7. 4. Leukocyte Adhesion Deficiency: an inherited defect in Mac-1,LFA-1, and p150,95 glycoproteins. Anderson, D.C. and Springer, T.A.. Ann. Rev. Med.1987;38:175. 5. Two pathways of CD11b/CD18-mediated neutrophil aggregation with different involvement of protein kinase C-dependent phosphorylation. Merrill, J.T., Slade, S.G., Weissman, G., Winchester, R., Buyon, J.P.. J. Immunology. 1990 Oct.1;145(8):2608-15. 6. Tumor necrosis factor-induce degranulation in adherent human neutrophils is dependent on CD11b/CD18-integrin -triggered oscillations of cytosolic free Ca2+. Richter, J., Ng-Sikorski, J., Olsson, I., Andersson, T.. Proc. Natl. Acad. Sci. USA, 1990 De;87(23):9472-6.
Related websites: https://www.medchemexpress.com/antibodies.html
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