Product Name :
Mouse anti Human CD27, conjugated with PE

Description :
| Clone DEN-3 | Isotype IgG1 | Product Type Single-Color Reagent | Units 100 Tests | Host Mouse | Species Reactivity Human | Application Flow Cytometry

Background :

Source :
The CD27 antigen recognizes disulfide-linked dimer nerve growth factor (NGF) super family of cells expressing the 55kD surface antigen. CD27 acts as a co-stimulatory on T lymphocytes with its ligand of CD70. Synonyms: CD27PE <

Product :
Product Form: RPE Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein Purification Method: Protein A/G Chromatography Concentration: Titered for flow cytometry

Specificity :

Applications :
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. Functional Analysis: Flow Cytometry Staining

Storage :
Product should be stored at 4-8°C. DO NOT FREEZE Product Stability: See expiration date on vial Shipping Conditions: Room Temperature

Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References :
1. Morimoto C., Cluster report : CD27. In Schlossman SF., Boumsell L., Gilks W., et al., eds. Leukocyte Typing V: White Cell Differentiation Antigens. Oxford: Oxford University Press: 1995:356-357. 2. CD27 induction on thymocytes. J Immunol 1990 Sep ;145(5):1356-63 Martorell J; Rojo I; Vilella R; Martinez-Caceres E; Vives J 3. CD27 expression by a distinct subpopulation of human B lymphocytes. Eur J Immunol 1990 De;20(12):2679-84 Maurer D; Holter W; Majdic O; Fischer GF; Knapp W 4. Regulation of expression of CD27, a T cell-specific member of a novel family of membrane receptors. J Immunol 1991 Apr 1;146(8):2488-94 de Jong R; Loenen WA; Brouwer M; van Emmerik L; de Vries EF; Borst J; van Lier RA 5. The T-cell activation antigen CD27 is a member of the growth factor/tumor necrosis factor receptor gene family. Camerini D., Walz G., Loenen W., Borst J., Seed B. J. Immunol. 1991:147(9):3165-3169

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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