Product Name :
Mouse anti Human CD28

Description :
| Clone B-23 | Isotype IgG1 | Product Type Single-Color Reagent | Units 100 µg | Host Mouse | Species Reactivity Human | Application Flow Cytometry

Background :
Derived from the hybridization of mouse Sp2/O-Ag14 myeloma cells with spleen cells of BALB/c mice immunized with the HPB-ALL T-cell line.

Source :
Anti-human CD28 binds the 44kDa MW cell surface protein on the surface of most T cells. CD28 acts as the ligand for the B7/BB-1 molecule on the surface of activated B cells. B7/BB-1 costimulates T cells through CD28, along with CD2 and CD3.CD28 antigen is a disulfide-linked homodimeric glycoprotein. The CD28 antigen is present on approximately 60%-80% T lymphocytes (95% of CD4 and 50% of CD8 lymphocytes). CD28 regulates the expression of cytokines by T cells, not only IL-2, but also IL-1 alpha and CSF-1, usually synthesized by accessory cells. CD28 functions as a cell adhesion molecule (CAM) for certain T cell subsets. Synonyms: CD28 <

Product :
Provided as solution in phosphate buffered saline with 0.08% sodium azide Product Form: Unconjugated Purification Method: Protein A/G Chromatography Concentration: See vial for concentration

Specificity :

Applications :
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2° to 8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. Functional Analysis: Flow Cytometry Staining

Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature

Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References :
1. CD28 is an inducible T cell surface antigen that transduces a proliferative signal in CD3+ mature thymocytes. Turka LA; Ledbetter JA; Lee K; June CH; Thompson CB J Immunol 1990 Mar ;144(5):1646-53 2. CD28 ligation in T-cell activation: evidence for two signal transduction pathways. Ledbetter JA; Imboden JB; Schieven GL; Grosmaire LS; Rabinovitch PS; Lindsten T; Thompson CB; June CH Blood 1990 Apr ;75(7):1531-9 3. T-cell antigen CD28 mediates adhesion with B cells by interacting with activation antigen B7/BB-1. Linsley PS; Clark EA; Ledbetter JA Proc Natl Acad Sci U S A 1990 Ju;87(13):5031-5 4. Role of the CD28 receptor in T-cell activation. June CH; Ledbetter JA; Linsley PS; Thompson CB Immunol Today 1990 Ju;11(6):211-6 5. Differences in surface phenotype and mechanism of action between alloantigen-specific CD8+ cytotoxic and suppressor T cell clones. Koide J; Engleman EG J Immunol 1990 Jan ;144(1):32-40 6. Binding of the B cell activation antigen B7 to CD28 costimulates T cell proliferation and interleukin 2 mRNA accumulation. Linsley PS; Brady W; Grosmaire L; Aruffo A; Damle NK; Ledbetter JA J Exp Med 1991 Mar ;173(3):721-30 7. The CD28 ligand B7/BB1 provides costimulatory signal for alloactivation of CD4+ T cells. Koulova L; Clark EA; Shu G; Dupont B J Exp Med 1991 Mar ;173(3):759-62

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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