Product Name :
Mouse anti Human CD4 FITC – HLADR PE

Description :
| Product Type Bi-Testª Reagents (FITC/RPE) | Units 50 Tests | Host Mouse | Species Reactivity Human | Application Flow Cytometry

Background :
Immunogen: CD4=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.

Source :
CD4 identifies human helper/inducer T cells expressing the 60,000 M.W. surface antigen (HLA class II reactive). CD4 is present in low density on monocytes. HLA-DR identifies human B cell and T cell subsets associated with approximately 10% of peripheral blood lymphocytes 28-34,000 M.W. surface antigen, also low density on monocytes and macrophages. Synonyms: CD4 FITC – HLADR PE <

Product :
Product Form: Bi-Test (FITC/RPE) Reagent Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein Purification Method: Protein A/G Chromatography Concentration: Titered for flow cytometry

Specificity :

Applications :
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2° to 8°C. Wash twice with PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20°C. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. Functional Analysis: Flow Cytometry Staining

Storage :
Product should be stored at 4-8°C. DO NOT FREEZE Product Stability: See expiration date on vial Shipping Conditions: Room Temperature

Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References :
1. Thymus Dependent Membrane Antigens in Man: Inhibition of Cell-Mediated Lympholysis by Monoclonal Antibodies to the TH-2 Antigen. Evans, R.L., Wall, D.W., Platsoucas, C.D., Siegal, F.P., Fikrig, S.M., Testa, C.M, and Good, R.A.. Proc. Nat. Acad. Sci. 78,544,1981. 2. Novel Immunoregulatory Functions of Phenotypically Distinct Subpopulations of CD4+ cells in the Human Neonate. Clement, L.T., Vink, P.E., Bradley, G.E.. J. Immunology 145(1):102-8,1990. 3. Antigen Presentation by the CD4 Positive Monocyte Subset. Szabo, G., Miller, C.L., Kodys, K.. J. Leukoc. Biol. 47(2): 11120,1990. 4. Human Immunodeficiency Virus Infection is Efficiently Mediated by a Glycolipid-Anchored form of CD4. Diamond, D.C., Finberg, R., Chaudhuri, S., Sleckman, B.P., Burakoff, S.J.. Proc. Natl. Acad. Sci. 87(13):5001-5,1990. 5. Development Regulation of the Intrathymic T cell Precursor Population. Adkins, B., J.. Immunol. 146(5):1387-93,1991. 6. Induction of CD4 and Susceptibility to HIV-1 Infection in Human CD8+ T Lymphocytes by Human Herpesvirus 6. Lusso, P., De Maria, A., Malnati, M., Lori, F., DeRocco, S.E., Baseler, M., Gallo, R.C.. Nat. 349(6309):533-5,1991. 7. Structural requirements for pairing of alpha and beta chains in HLA-DR and HLA-DP molecules. Karp, D.R., Teletski, C.L., Jaraquemada, D., Maloy, W.L., Coligan, J.E., Long, E.O., J. Exp.. Med. 1990 March ;171(3):615-28. 8. Trans-activation of HLA-DR gene by hepatitis B virus X gene product. Hu K.Q., Vierling J.M., Siddiqui A.. Proc. Natl. Acad. Sci. USA 1990 SEPT.; 87(18):7140-4. 9. Purified primitive human hematopoietic progenitor cells with long-term in vitro repopulating capacity adhere selectively to irradiated bone marrow stroma. Verfaillie C., Blakolmer K., McGlave P., J. Exp. Med. 1990 Aug.: 172(2):509-2. 10. Increased circulating HLA-DR+ CD4+ T cells in systemic lupus erythematosus: alterations associated with prednisolone therapy. Raziuddin, S., Nur, M.A., Al-Wabel, A.A., Scand., Immunol. 1990 Feb.:31(2):139-45.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
Related websites:
Popular product recommendations:
GPR43 Antibody
AKT1 Antibody (YA834)
Calcitonin receptor Antibody: Calcitonin receptor Antibody is an unconjugated, approximately 53 kDa, rabbit-derived, anti-Calcitonin receptor polyclonal antibody. Calcitonin receptor Antibody can be used for: ELISA, IHC-P, IHC-F, ICC, IF expriments in rat, and predicted: mouse background without labeling.