Product Name :
Mouse anti Human CDC25A

Description :
| Clone DCS121 | Isotype IgG2a | Product Type Monoclonal Antibody | Units 100 µg | Host Mouse | Species Reactivity Human | Application Immunoprecipitation Western Blotting

Background :
Mice were immunized with a purified recombinant CDC25A protein of 65kD protein

Source :
The immunogen for the anti CDC25A was a purified recombinant CDC25A protein recognizing a 65kD protein. In the cell cycle of eukaryotes the M phase is initiated by maturation promoting factor (MPF), a protein kinase complex consisting of p34 cdc2, cyclin B, and p13. The activity of this complex is controlled by multiple levels of phosphorylation and dephosphorylation of the catalytic p34cdc2 component. CDC25A and CDC25B arep34cdc2 tyrosine phosphates. Dephosphorylation of p34cdc2 by these CDC25 proteins activates the MPF complex, initiating mitosis. Although expressed throughout the cell cycle, peak expression of CDC25A is in G1 of the cell cycle. Synonyms: Dual specificity phosphatase Cdc25A; M-phase inducer phosphatase 1; MPIP1; <

Product :
Product Form: Unconjugated Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide Purification Method: Protein A/G Chromatography Concentration: See vial for concentration

Specificity :

Applications :
Western Blot, Immunoprecipitation at 1-10 ug/ml Functional Analysis: Western Blotting

Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature

Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References :
1. Wang, Z., et al. ‘Overexpression of CDC25B, CDC25C and phospho-CDC25C (Ser216) in vulvar squamous cell carcinomas are associated with malignant features and aggressive cancer phenotypes.’ BMC Cancer 2010, 10, 233 2. Berthet, C., et al. ‘How adeno-associated virus Rep78 protein arrests cells completely in S phase.’ Proc. Natl. Acad. Sci. 2005, 102, 13634-13639

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
Related websites:
Popular product recommendations:
COX2 Antibody
beta III Tubulin Antibody: beta III Tubulin Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 50 kDa, targeting to beta III Tubulin. It can be used for WB,IHC-F,IHC-P,ICC/IF,IP assays with tag free, in the background of Human, Mouse, Rat.