Product Name :
Mouse anti Human Insulin

Description :
| Clone E2E3 | Isotype IgG1 | Product Type Monoclonal Antibody | Units 100 µg | Host Mouse | Species Reactivity Bovine Human Porcine | Application ELISA Immunohistochemistry Western Blotting

Background :
Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with full-length insulin protein and mouse myeloma cells.

Source :
Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma. <

Product :
Product Form: Unconjugated Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide Purification Method: Protein A/G Chromatography Concentration: See vial for concentration

Specificity :

Applications :
Antibody can be used for Western blotting (1-5 µg/ml), immunohistochemistry on formalin-fixed, paraffin-embedded tissues (1-2 µg/ml, incubate at room temp. for at least 1 hr.) and ELISA. Optimal concentration should be evaluated by serial dilutions. Functional Analysis: Western Blotting

Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References :
1. Roth, J., et al. ‘Distribution patterns of proinsulin and insulin in human insulinomas: an immunohistochemical analysis in 76 tumors.’ Virchows Arch B Cell Pathol Incl Mol Pathol 63, 51-61 (1992). 2. Nykiforuk, C.L., et al. ‘Transgenic expression and recovery of biologically active recombinant human insulin from Arabidopsis thaliana seeds.’ Plant Biotechnology J. 4, 77-85 (2006) 3. Lee, A.H., et al. ‘XBP-1 is required for biogenesis of cellular secretory machinery of exocrine glands.’ EMBO J., 24, 4368-4380 (2005)

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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