Product Name :
Mouse anti Human Keratin (4,5,6,8,10,13,18)

Description :
| Clone C11 | Isotype IgG1 | Product Type Monoclonal Antibody | Units 100 µg | Host Mouse | Species Reactivity Human | Application Immunohistochemistry (frozen & paraffin) Western Blotting

Background :
Hybridoma produced by the fusion of splenocytes from mice immunized with cytoskeleton preparation from human A431 carcinoma cells and mouse myeloma cells.

Source :
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. <

Product :
Product Form: Unconjugated Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide Purification Method: Purified by saturated ammonium sulphate precipitation Concentration: See vial for concentration

Specificity :

Applications :
X1260M detects keratins 4,5,6,8,10,13 & 18 by Western blot. Immunohistochemistry can be performed on frozen tissues and paraffin sections. Optimal concentration should be evaluated by serial dilutions. X1260M reacts with a variety of normal and neoplastic epithelia. Reacting with simple epithelium and both basal and superbasal layers of cornifying and non-cornifying squamous epithelium this antibody is also useful in staining cultured epithelial cell lines. It is useful in differentiating epithelial tumors from non-epithelial tumors. Functional Analysis: Western blotting

Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References :
1. Folia Biol. 1989, 35, 373-382 2. Neoplasma 1990, 37, 333-342 3. J. Tumor Marker Oncol. 1990, 5, 219 4. Int. J. Cancer 1988, Suppl. 3, 50-55

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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