Product Name :
Mouse anti Human Keratin 7 (7, 17)
Description :
| Clone C46 | Isotype IgG1 | Product Type Monoclonal Antibody | Units 100 µg | Host Mouse | Species Reactivity Human | Application Immunohistochemistry (frozen) Western Blotting
Background :
Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with cytoskeleton preparation from HeLa cells and mouse myeloma cells.
Source :
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. <
Product :
Product Form: Unconjugated Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide Purification Method: Protein A/G Chromatography Concentration: See vial for concentration
Specificity :
Applications :
Detects keratin 7 and 17 by Western blot. Detects a 54 kDa band, corresponding to keratin 7, and a 46 kDa band, corresponding to keratin 17. Reacts only with keratin 7 in formalin-fixed paraffin-embedded sections. Optimal concentration should be evaluated by serial dilutions. Functional Analysis: Western Blotting
Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
Neoplasma 1990, 37, 333-342
Related websites: https://www.medchemexpress.com/antibodies.html
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