Product Name :
Mouse anti Human Matrix Metalloproteinase 9 (MMP9)
Description :
| Clone 4A3 | Isotype IgG1 | Product Type Monoclonal Antibody | Units 200 µg | Host Mouse | Species Reactivity Human | Application Immunohistochemistry (paraffin) Western Blotting
Background :
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the N-terminus of the human MMP9 protein and mouse myeloma Ag8563 cells. Sequence common in rabbit, dog and pig
Source :
The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-9 (also designated 92-kDa type IV collagenase or gelatinase B) has been shown to degrade bone collagens in concert with MMP-1 (also designated interstitial collagenase, fibroblast collagenase or collagenase-1), and cysteine proteases and may play a role in bone osteoclastic resorption. MMP-1 is down-regulated by p53, and abnormality of p53 expression may contribute to joint degradation in rheumatoid arthritis by regulating MMP-1 expression. Synonyms: CLG4B, GELB, 92 kDa gelatinase, matrix metallopeptidase 9 <
Product :
Provided as solution in phosphate buffered saline with 0.08% sodium azide Product Form: Unconjugated Purification Method: Protein A/G Chromatography Concentration: See vial for concentration
Specificity :
Applications :
Antibody can be used for Western blotting (1-2 µg/ml) and immunohistochemistry (1-5 µg/ml). Antibody is specific for the activated form of MMP9 only. These working concentrations are merely an indication. Optimal working concentrations should, however, be evaluated by serial dilutions by the customer. Functional Analysis: N.A. Positive Control: Esophageal adenocarcinoma. Only present in tumor tissues.
Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
1. Wang, L. et al. (2006). Matrix metalloproteinase 2 (MMP2) and MMP9 secreted by erythropoietin-activated endothelial cells promote neural progenitor cell migration. J. Neurosci. 26(22);5996-6003 2. Solmiari, S.B., et al. (2006). Circulating MMP2 and MMP9 in breast cancer — potential role in classification of patients into low risk, high risk, benign disease and breast cancer categories. Int. J. Cancer. 119(6);1403-1411 3. Chen, X., et al. (2005). Increased plasma MMP9 in integrin alpha1-null mice enhances lung metastasis of colon carcinoma cells. Int. J. Cancer. 116(1);52-61
Related websites: https://www.medchemexpress.com/antibodies.html
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