Product Name :
Mouse anti Human Muc1
Description :
| Clone VU-3C6 | Isotype IgG1 | Product Type Monoclonal Antibody | Units 100 µg | Host Mouse | Species Reactivity Human | Application ELISA Immunohistochemistry (frozen & paraffin) Western Blotting
Background :
Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with live human breat cancer cells (cell line ZR-75-1) and mouse myeloma SP2 cells.
Source :
Muc1 is a heavily O-glycosylated transmembrane protein expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed in >90% breast carcinomas and metastases. In normal mammary gland it is expressed in apical surface of glandular epithelium. In breast cancer Muc1 is overexpressed; is underglycosylated and the apical localization is lost. Muc1 is transcribed as a larger precursor which is cleaved to form a larger mucin like subunit (265-400kDa) and a smaller subunit (14-28kDa) noncovalently associated with each other. Transgenic Muc1 has been shown to associate with all four erbB receptors and localize with erbB1 (EGFR) in lactating glands1. Muc1 can act as a ligand for ICAM-1 on HUVEC cells; it can bind -catenin, GSK3 and it associates with Grb2-SOS upon phosphorylation. Synonyms: Episialin <
Product :
Product Form: Unconjugated Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide Purification Method: Protein A/G Chromatography Concentration: See vial for concentration
Specificity :
Applications :
The antibody reacts with the extracellular 12-mer GVTSAPDTRPAP epitope of the MUC-1 tandem repeat.Antibody can be used for immunohistochemistry on frozen and paraffin embedded tissues and ELISA. Optimal concentration should be evaluated by serial dilutions. Functional Analysis: Western Blotting Positive Control: MCF-7 and T47D cells
Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
1. Schol, D.J. et al., Epitope fingerprinting using overlapping 20-mer peptides of the MUC 1 tandem repeat sequence. Tumor Biol. Suppl. 1998: 19, 35-45. 2. Price, M.R. et al., Analysis of 56 monoclonal antibodies against the MUC1 mucin.Tumor Biol. Suppl. 1998: 19, 1-20. 3. Cao, Y. et al., Immunohistochemical characterization of a panel of 56 anti bodies with normal human small intestine, colon, and breast tissues. Tumor Biol. Suppl. 1998: 19, 88-99
Related websites: https://www.medchemexpress.com/antibodies.html
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