Product Name :
Mouse anti Human T-Complex Protein 1, subunit
Description :
| Clone F39 P7 F11 | Isotype IgG2a | Product Type Monoclonal Antibody | Units 200 µg | Host Mouse | Species Reactivity Frog Human Mouse Rat | Application Immunohistochemistry Western Blotting
Background :
Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the C-terminal of the TCP-1 protein and mouse myeloma Ag8563 cells. Sequence common in human, frog, mouse, rat
Source :
The protein TCP-1 (t-complex polypeptide 1) is a subunit of the hetero-oligomeric complex CCT (chaperonin containing TCP-1) present in the eukaryotic cytosol. The CCT of eukaryotic cytosol is composed of eight different subunit species that are proposed to have independent functions in folding its in vivo substrates, the Actins and Tubulins. TCP-1 was first identified in the mouse as relevant for tail-less and embryonic lethal phenotypes. Sequences homologous to TCP-1 have been isolated in several other species, and the yeast TCP-1 has been shown to encode a molecular chaperone for Actin and Tubulin. TCP-1 found in mammalian cells and yeast plays an important role in the folding of cytosolic proteins. Synonyms: TCP-1b; Chaperonin containing TCP1, subunit 2 (beta); CCT-beta; CCTB <
Product :
Product Form: Unconjugated Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide Purification Method: Protein A/G Chromatography Concentration: See vial for concentration
Specificity :
Applications :
Antibody can be used for Western blotting (1-2 µg/ml) and immunohistochemistry on formlin-fixed, paraffin-embedded tissue sections (1-5µg/ml). Optimal concentration should be evaluated by serial dilutions. Functional Analysis: Western Blotting Positive Control: Colorectal cancer tissue, Calu6 and HT29 cell lysates
Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
1. Kubota, H., et al. (1999). Structures and co-regulated expression of the genes encoding mouse cytosolic chaperonin CCT subunits. Eur. J. Biochem. 262(2):492-500 2. Joly, E.C., et al. (1994). cDNA encoding a novel TCP1-related protein. Biochm. Biophys. Acta. 1217(2):224-226
Related websites: https://www.medchemexpress.com/antibodies.html
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