Product Name :
Mouse anti Phosphotyrosine, conjugated with biotin

Description :
| Clone PY20 | Isotype IgG2b | Product Type Monoclonal Antibody | Units 100 µg | Host Mouse | Species Reactivity Ubiquitous | Application Enzyme Immunoassay Immunohistochemistry Immunoprecipitation Western Blotting

Background :
Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.

Source :
The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine-phosphorylated proteins biochemical pathways in which they function. Synonyms: Mouse anti phosphotyrosine, PY20, Biotin Conjugate <

Product :
Product Form: Biotin Formulation: Provided as a solution containing PBS, 65% glycerol and 0.08% sodium azide. Purification Method: Prot A/G Concentration: See vial for concentration

Specificity :

Applications :
The biotin conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of phosphotyrosyl proteins in western blotting, immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would produce unacceptable background signals. In addition, the strength of the biotin-streptavidin interaction and the immense variety of streptavidin-coupled probes available make PY20-biotin a very versatile reagent. A dilution of 1:5,000 is suggested for western blot or ELISA, 1:500 for immunohistochemistry or immunocytochemistry. Functional Analysis: Western Blotting

Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References :

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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