Product Name :
Mouse anti Vimentin

Description :
| Clone V9 | Isotype IgG1 | Product Type Primary Antibodies | Units 0.1 mg | Host Mouse | Species Reactivity Chicken Human Rat Sheep | Application Flow Cytometry Immunocytochemistry Immunohistochemistry (frozen) Immunohistochemistry (paraffin) Western Blotting

Background :
V9 is a Mouse monoclonal IgG1 antibody derived by fusion of PAI Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with vimentin isolated from porcine lens.

Source :
Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP, often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. <

Product :
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide. Formulation: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Specificity :
V9 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. Species Reactivity: Also reacts with potoroo

Applications :
V9 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues and flow cytometry. For staining paraffin-embedded tissues pretreatment by boiling tissue sections for 10 minutues in 10 mM citRate buffer (pH 6.0) is required. Optimal antibody dilution should be determined by titration; recommended range is 1:25 – 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.

Storage :
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References :
1. Osborn, M., Debus, E., and Weber, K. (1984). Monoclonal antibodies specific for vimentin, Eur J Cell Biol 34, 137-43. 2. Tolle, H. G., Weber, K., and Osborn, M. (1985). Microinjection of monoclonal antibodies specific for one intermediate filament protein in cells containing multiple Keratins allow insight into the composition of particular 10 nm filaments, Eur J Cell Biol 38, 234-44. 3. Van Muijen, G. N., Ruiter, D. J., and Warnaar, S. O. (1987). Coexpression of intermediate filament polypeptides in Human fetal and adult tissues, Lab Invest 57, 359-69. 4. Van Muijen, G. N., Warnaar, S. O., and Ponec, M. (1987). Differentiation-related changes of Cytokeratin expression in cultured Keratinocytes and in fetal, newborn, and adult epidermis, Exp Cell Res 171, 331-45. 5. Corver, W. E., Koopman, L. A., van der Aa, J., Regensburg, M., Fleuren, G. J., and Cornelisse, C. J. (2000). Four-color multiparameter DNA flow cytometric method to study phenotypic intratumor heterogeneity in cervical cancer, Cytometry 39, 96-107. 6. De Visscher, G., Plusquin, R., Mesure, L., Flameng, W. (2010). Selection of an immunohistochemical panel for cardiovascular research in Sheep. Appl Immunohistochem Mol Morphol 18, 382-91.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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