Product Name :
Rabbit anti Human Acid Ceramidase 1
Description :
| Isotype IgG | Product Type Antigen Immunoaffinity Purified Polyclonal | Units 100 µg | Host Rabbit | Species Reactivity Human Mouse Rat | Application ELISA Immunohistochemistry Western Blotting
Background :
Immunogen: Synthetic peptide derived from the C terminal region of the ASAH1 protein.
Source :
Acid ceramidase is a lipid hydrolyase responsible for the degradation of ceramide into sphingosine and free fatty acids within lysosomes. It can also synthesize ceramide from sphingosine and free fatty acids as well. The reverse activity is pH dependent (6.0 vs 4.5, respectively). This suggests that the enzyme may have diverse functions within cells dependent on its subcellular location and the local pH. Recent studies have shown that acid ceramidase activity is aberrantly expressed in several human cancers and that it might be a useful drug target. As an example, inhibitors of enzyme activity have been shown to slow the growth of cancer cells alone or in combination with other established, anti-oncogenic treatments. Aberrant activity has also been shown in AlzheimerÕs disease and overexpression may prevent insulin resistant (Type II) diabetes by free fatty acids. Synonyms: N-acylsphingosine amidohydrolase (Acid ceramidase) 1; ASAH1 <
Product :
Product Form: Unconjugated Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide Purification Method: Antigen Immunoaffiinity Purification Concentration: See vial for concentration
Specificity :
Applications :
Antibody can be used for Western blotting (1-5 µg/ml) and Immunohistochemistry on paraffin-embedded tissues (10 µg/ml). Optimal concentration should be evaluated by serial dilutions. Functional Analysis: Western Blotting Positive Control: Human heart tissue
Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
1. Park, J.H., et al. ‘Acid ceramidase and human disease.’ Biochim Biophys Acta 2006; 1758:2133-2138. 2. Zeidan, Y.H., et al. ‘Acid ceramidase but not acid sphingomyelinase is required for tumor necrosis factor-{alpha}-induced PGE2 production.’ J. Biol. Chem. 2006; 281:24695-24703. 3. Shtraizent, N., et al. ‘Autoproteolytic cleavage and activation of human acid ceramidase.’ J. Biol. Chem. 2008; 283:11253-11259.
Related websites: https://www.medchemexpress.com/antibodies.html
Popular product recommendations:
GAPDH Antibody
Rat IgG1 kappa, Isotype Control
Anti-Aurora A Antibody: Anti-Aurora A Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 46 kDa, targeting to Aurora. It can be used for WB, IF, IHC-P, FC assays with tag free, in the background of Human, Mouse, Rat.