Product Name :
Chicken anti Staphylococcal Protein A, conjugated with HRP

Description :
| Product Type Primary antibodies | Units 0.5 mg | Host Chicken | Species Reactivity Bacteria | Application ELISA Western Blotting

Background :
Chickens were immunized with purified recombinant protein A. After multiple immunizations in Freunds adjuvant, eggs were collected and the IgY fraction purified. Antibodies were immunoaffinity-purified from a protein A agarose column and conjugated to horseradish peroxidase (HRP).

Source :

Product :
Product Form: HRP conjugated Formulation: Phosphate Buffered Saline‚ 50% Glycerol with 0.1% 2-Chloroacetamide Purification Method: Egg-yolk derived IgY Affinity Purified Concentration: See vial for concentration.

Specificity :

Applications :
This antibody is suitable for ELISA and immuoblotting. Recommended starting dilutions for ELISA capture is 1:100 – 1:500 and for blotting, 1:5,000 – 1:20,000.

Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles. Shipping Conditions: Ship at ambient temperature

Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References :
1. Freiherr von Roman M1, Koller A, von Rüden D, Berensmeier S. 2014. Improved extracellular expression and purification of recombinant Staphylococcus aureus protein A. Protein Expr Purif. 93:87-92

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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