Product Name :
Rabbit anti-NKG2D Polyclonal Antibody
Synonym :
KLRK1; natural killer cell group 2D; NKG2-D isoform b; Klrk1; Nkrp2; NKG2-D type II integral membrane protein; NKG2-D-activating NK receptor; NK cell receptor D; Killer cell lectin-like receptor subfamily K; NK lectin-like receptor; CD314; CD314 antigen; D12S2489E; Killer cell lectin like receptor subfamily K member 1; Killer cell lectin-like receptor subfamily K member 1; KLR; NKG2 D activating NK receptor; NKG2 D type II integral membrane protein; Nkg2d; NKG2D_HUMAN; NKLLR; NKR P2
Host :
Rabbit
Species Reactivity:
Mouse
Specificity :
NKG2D
Predicted Reactivity:
Human, Rat
Applications :
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500
Immunogen:
KLH conjugated synthetic peptide derived from human NKG2D:121-219/219
Concentration :
1mg/ml
Purification :
affinity purified by Protein A
Clonality:
Polyclonal Antibody
Storage Temp.:
Store at -20 ° C for one yearAvoid repeated freeze/that cycles
Research areas :
Cell biology Immunology
Background :
This locus represents naturally occurring read-through transcription between the neighboring KLRC4 (killer cell lectin-like receptor subfamily C, member 4) and KLRK1 (killer cell lectin-like receptor subfamily K, member 1) genes on chromosome 12. The read-through transcript includes an alternate 5′ exon and lacks a significant portion of the KLRC4 coding sequence, including the start codon, and it thus encodes the KLRK1 protein. [provided by RefSeq, Dec 2010]
UniProt :
O70215
Additional information:
Product Details FAQ Citations(0) Video Pictures Documents |Overview |Synonym KLRK1; natural killer cell group 2D; NKG2-D isoform b; Klrk1; Nkrp2; NKG2-D type II integral membrane protein; NKG2-D-activating NK receptor; NK cell receptor D; Killer cell lectin-like receptor subfamily K; NK lectin-like receptor; CD314; CD314 antigen; D12S2489E; Killer cell lectin like receptor subfamily K member 1; Killer cell lectin-like receptor subfamily K member 1; KLR; NKG2 D activating NK receptor; NKG2 D type II integral membrane protein; Nkg2d; NKG2D_HUMAN; NKLLR; NKR P2 |Host Rabbit |Specificity NKG2D |Species Reactivity Mouse |Predicted Reactivity Human, Rat |Applications WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 |Immunogen KLH conjugated synthetic peptide derived from human NKG2D:121-219/219 |Properties |Concentration 1mg/ml |Purification affinity purified by Protein A |Clonality Polyclonal Antibody |Isotype IgG |Storage Temp. Store at -20 ° C for one yearAvoid repeated freeze/that cycles |Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |Research areas Cell biology Immunology |Target |Background This locus represents naturally occurring read-through transcription between the neighboring KLRC4 (killer cell lectin-like receptor subfamily C, member 4) and KLRK1 (killer cell lectin-like receptor subfamily K, member 1) genes on chromosome 12. The read-through transcript includes an alternate 5′ exon and lacks a significant portion of the KLRC4 coding sequence, including the start codon, and it thus encodes the KLRK1 protein. [provided by RefSeq, Dec 2010] |Cellular localization Cell membrane; |UniProt O70215 | Sample: Lung (Mouse) Lysate at 40 ugPrimary: Anti- NKG2D (abs120298) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 25 kDObserved band size: 26 kD| Blank control (blue line): Mouse thymus cells (blue). Primary antibody: Rabbit Anti-NKG2D antibody Dilution: 1μg /10^6 cells; Isotype Control antibody: Rabbit IgG . Secondary antibody: Goat anti-rabbit IgG-PEDilution: 1μg /test. ProtocolThe cells were fixed with 70% methanol (Overnight at 4℃) . Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |Tips:This product is for research use only. Not for use in diagnostic prodcedures.
Rabbit anti-NKG2D Polyclonal Antibody
Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.Related websites: https://www.medchemexpress.com/antibodies.html
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