Ies of cardiovascular toxicity and assist in tailoring the danger management of personal sufferers. Funding: This task was funded through the Princess Margaret Cancer Centre.PS03.Extracellular vesicles derived from genetically modified human induced pluripotent stem cells enrich cardiomyogenesis and angiogenesis in vitro and in vivo Katarzyna Kmiotek-Wasylewska, Sylwia Bobis-Wozowicz, Anna LabedzMaslowska, PKD3 custom synthesis Elzbieta Karnas, Zbigniew Madeja and Ewa Zuba-Surma Division of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, PolandIntroduction: In spite of their efficacy as an anti-cancer therapeutic towards chronic myelogenous leukaemia (CML), tyrosine kinase inhibitors (TKIs) is often connected with deleterious cardiovascular effects. Significant progress has become created in identifying the excess chance of cardiovascular events related to TKI publicity; on the other hand, the data over the underlying mechanisms and doable predictive biomarkers are currently inadequate. To this end, we sought to examine EV-associated miRNAs being a usually means of elucidating their likely as effectors and biomarkers of TKIinduced cardiovascular toxicity in CML. Approaches: We obtained informed consent and recruited 24 age- and sex-matched response stable CML individuals both off-TKI (median 32.26 months, n = 6) or on long-term remedy with imatinib, nilotinib or ponatinib (median 79.01 months, n = 6/group), and assayed plasma-derived EV-associated miRNAs using the nCounterAnalysis Technique. Concurrently, in vitro scientific studies were carried out to examine the responses of iPSCderived human cardiomyocytes to plasma-derived EVs applying BNP being a surrogate marker of the cardiovascularIntroduction: Extracellular vesicles (EVs) represent population of tiny circular membrane vesicles secreted by most cells such as stem cells (SCs). It has been reported that EVs could carry bioactive cargo which includes proteins, microRNAs and mRNAs. Additionally they perform a important part in cell-to-cell communication in each physiological and pathological problems. The aim of this study was to confirm the influence of EVs derived from human induced pluripotent stem (iPS) cells (hiPS-EVs) overexpressing procardiomyogenic miR1 or miR199a, or proangiogenic miR126, on a variety of properties of human Cardiac and endothelial cells. Techniques: hiPS-EVs were isolated from conditioned hiPS culture media by differential centrifugation which include ultracentifugation. Cardiac cells and endothelial cells have been utilised as target cells in vitro, and their functional properties were evaluated after hiPSEVs treatment. The regenerative capacity of hiPS-EVsISEV2019 Nav1.3 Synonyms ABSTRACT BOOKwas also examined in vivo in murine model of acute limb ischaemia (LI). Success: Our information indicate that hiPS-EVs carrying procardio- and proangiogenic miRNAs may perhaps defend cardiac cell types from apoptosis at the same time as enhance their proliferation, metabolic activity, migration and cardiomyogenic differentiation. The hiPS-EVs enhanced also proangiogenic capacity, migration and metabolic activity of HCAEC cells in vitro. The vesicles also promoted angiogenesis and improved blood flow recovery in murine ischaemic limb damage model in vivo. Summary/Conclusion: These final results might indicate (i) feasibility of genetic modifications of EVs enforcing their regenerative proprieties as well as (ii) enhanced action of EVs from hiPS cells overexpressing miR1, miR199a and miR126 in regeneration of ischaemic tissues. We conclude that EVs from genetically modified.