assessed for acute toxicity by means of various parameters in embryos and adult animals. Finally, we performed in silico analysis through the SEA server and docking working with the computer software GOLD. The phytochemical study showed the compounds cornoside, dihydrocornoide, and 1-O-methyl-myoinositol (bornesitol). The D2 Receptor Modulator review therapy with all doses of LxHs substantially decreased alloxan-induced hyperglycemia with no any substantial histological or biochemical abnormalities. No substantial frequency of teratogenesis was observed within the embryos exposed for the extract, and no substantial behavioral modifications or deaths have been observed in adult animals. In silico, the outcomes showed a possible interaction among inositol and enzymes involved in carbohydrates’ metabolism. All round, the outcomes show a hypoglycemic activity of the extract in vivo, with no apparent toxicity. The computational studies recommend this could possibly be no less than partially as a result of presence of bornesitol, considering that inositols can interact with carbohydrates’ enzymes. Search phrases: Hancornia speciosa Gomes; 1-O-methyl-myoinositol; diabetes; zebrafishPharmaceuticals 2021, 14, 856. 2021, 14,two of1. Introduction Plants are utilised for therapeutic purposes within the healing or therapy of a selection of ailments. However, a substantial aspect of conventional makes use of isn’t supported by scientific research [1]. In Brazil, the usage of plants is widespread in folk medicine inside classic communities. Certainly one of these plants is Hancornia speciosa Gomes, popularly referred to as “mangabeira”. Native to this nation, this species is located throughout the Amazon forest, the Brazilian semiarid area (called “caatinga”), and also the Atlantic forest [2]. Despite its ecological, conventional, and analysis value, this species is currently endangered [3]. Phytochemical studies happen to be performed with unique tree parts, including leaves, barks, fruit, and latex. The leaves of H. speciosa Gomes are reported to have terpenoids, steroids, tannins [4,5], and xanthines [5]. Inside the CA I Inhibitor site fruits are found phenols (flavonoids, condensed tannins) and alkaloids [6,7]. Moraes and coworkers reported flavonoids and tannins (proanthocyanidins) within the barks’ ethanol extract [8]. In 2016, Neves and coworkers reported the occurrence of phenols within the tree’s latex [9]. In literature, reports indicate angiogenic and osteogenic possible inside the latex, without the need of cytotoxicity or genotoxicity [92]. While this latex is related for the seringueira’s latex (Hevea brasiliensis L.), the former is most generally utilised against tuberculosis, ulcer, fungi infection, and a few inflammatory conditions [13]. The latex of mangabeira is generally consumed mixed with water to treat inflammatory illnesses and also other situations; in spite of the latex’s frequent usage, research in the literature about its prospective are nonetheless lacking compared to the other three components [14]. The drug discovery process also relies on the security of compounds assessed, and zebrafish have been extensively utilized for this goal [157]. Diabetes mellitus (DM) is usually a syndrome resulting from dysfunctional metabolism of carbohydrates, fats, and proteins that could result in hyperglycemia–its most important feature [18]. The international raise in its prevalence tends to make it a public overall health burden worldwide [19]. In line with Miranda [20], the pathophysiology of diabetes is triggered by each genetic and environmental elements. Existing diabetes therapies meet many of the patients’ needs. Still, new ant