will report further investigations of the detoxification mechanism of fenquinotrione in rice inside a subsequent paper (manuscript in preparation). In conclusion, the results from the present study demonstrate that fenquinotrione controls a wide range of broadleaf and sedge weeds by inhibiting HPPD. Additionally, it’s assumed that the excellent rice selectivity of fenquinotrione is because of its metabolism in rice plants. In current years, the cultivation of paddy rice, like forage rice and fermented rice roughage, has been promoted in Japan to expand the demand for rice and effectively use abandoned farmland, along with the cultivation and dissemination of high-yield rice varieties suitable for these purposes happen to be progressing. We think that fenquinotrione will meet the current demand for herbicides which will be applied to a variety ofcultivation systems and rice varieties.Electronic supplementary materialsThe on the net version of this short article consists of supplementary materials (Supplemental Methods, Supplemental Figs. S1 4, and Supplemental Table 1), which are accessible at jstage.jst.go.jp/browse/jpestics/.
lifeArticleThe Efficiency of HepG2 and HepaRG Systems through the Glass of Acetaminophen-Induced ToxicityTam Lorincz 1,two, , Veronika De 1, , Kinga Makk-Merczel 1,two, , D a Varga 1,two , P er Hajdin 1,two and Andr Szarka 1,two,3, Laboratory of Biochemistry and Molecular Biology, Division of Applied Biotechnology and Meals Science, Budapest University of Technologies and Economics, Szent Gell t t 4, H-1111 Budapest, Hungary; lo[email protected] (T.L.); [email protected] (V.D.); SIRT5 Storage & Stability [email protected] (K.M.-M.); [email protected] (D.V.); [email protected] (P.H.) Biotechnology Model Laboratory, Faculty of Chemical Technologies and Biotechnology, Budapest University of Technologies and Economics, Szent Gell t t four, H-1111 Budapest, Hungary Department of Molecular Biology, Institute of Biochemistry and Molecular Biology, Semmelweis University, H-1428 Budapest, Hungary Correspondence: [email protected]; Tel.: +36-1-463-3858 These authors contributed equally towards the operate.Citation: L rincz, T.; De , V.; o Makk-Merczel, K.; Varga, D.; Hajdin , P.; Szarka, A. The Functionality of HepG2 and HepaRG Systems by means of the Glass of Acetaminophen-Induced Toxicity. Life 2021, 11, 856. doi.org/ 10.3390/life11080856 Academic Editor: Claudio Brancolini Received: 19 July 2021 Accepted: 18 August 2021 Published: 21 AugustAbstract: Investigation of drug-induced liver injuries demands suitable in vivo and in vitro toxicological model systems. In our study, an attempt was produced to compare the hepatocarcinoma HepG2 and the stem cell-derived HepaRG cell lines both in two- and three-dimensional culture situations to find the most suitable model. Comparison in the liver-specific traits of these models was performed by means of the extent and mechanism of acetaminophen (APAP)-induced hepatotoxicity. Investigating the detailed mechanism of APAP-induced hepatotoxicity, different NOX4 Formulation precise cell death inhibitors had been utilized: the pan-caspase inhibitor zVAD-fmk and dabrafenib considerably protected each cell lines from APAP-induced cell death. Nevertheless, the recognized distinct inhibitors of necroptosis (necrostatin-1 and MDIVI) have been only efficient in differentiated HepaRG, which suggest a differential execution of activated pathways in the two models. By applying 3D culture solutions, CYP2E1 mRNA levels could possibly be elevated, but we failed to attain a important