Product Name :
Mouse anti Human CD2 FITC – CD20 PE

Description :
| Isotype IgG2a (F)/IgG1 (PE) | Product Type Bi-Testª Reagents (FITC/RPE) | Units 100 Tests | Host Mouse | Species Reactivity Human | Application Flow Cytometry

Background :
Immunogen: CD2=Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with t lymphocytes activated by mixed lymphocyte culture. CD20=Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with the LB lymphoblastoid cell line.

Source :
Identification of human T cells and subset of NK cells associated with the receptor for sheep erythocytes rosettes expressing the 45-50,000 M.W. surface antigen. Identification of human T lymphocytes in multiple stages of T cell development, including a major subset of mature peripheral T cell. Identification of CD20 on human B cells associated with approximately 10% of peripheral blood lymphocytes. Synonyms: CD2 FITC – CD20 PE <

Product :
Product Form: Bi-Test (FITC/RPE) Reagent Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein Purification Method: Protein A/G Chromatography Concentration: Titered for flow cytometry

Specificity :

Applications :
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8° C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20° C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Storage :
Product should be stored at 4-8°C. DO NOT FREEZE Product Stability: See expiration date on vial Shipping Conditions: Room Temperature

Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References :
1.An Improved Rosetting Assay for Detection of Human T Lymphocytes. Kaplan M.E., Clark C., J. Immunol. Methods 1974, 5,131 2.Structural and functional characterization of the CD2 immunoadhesion domain. Evidence for inclusion of CD2 in an alpha-beta protein folding class. Recny M.A., Neidhardt E.A., Sayre P.H., Ciardelli T.L., Reinherz E.L., J. Biol. Chem. 1990 May 2;265(15):85419 3. Partial deletions of the cytoplasm domain of CD2 result in a partial defect in signal transduction. Bierer B.E., Bogart R.E., Burakoff S.J., J. Immunol. 1990 Feb. :144(3):785 4. Functional CD2 mutants unable to bind to, or be stimulated by, LFA-3. Wolff H.L., Burakoff S.J., Bierer B.E., J. Immunol. 1990 Feb. 1;144(4):1215-20 5. Association of CD2 and CD45 on human T lymphocytes. Schraven B., Samstag Y., Altevogt P., Meuer S.C., Nature 1990 May ;345(6270):71-4 6. In vivo and in vitro expression of myeloid antigens on B-lineage acute lymphoblastic leukemia cells. Leukemia 1991 Ja;5(1):19-25 Hara J; Kawa-Ha K; Yumura-Yagi K; Kurahashi H; Tawa A; Ishihara S; Inoue M; Murayama N; Okada S. 7. Activation of dense human tonsilar B cells. Induction of c-myc gene expression via two distinct signal transduction pathways. J Immunol 1991 Feb ;146(3):846-53 White MW; McConnell F; Shu GL; Morris DR; Clark EA. 8. Differential effects of low and high concentrations of interleukin 6 on human B cells. Eur J Immunol 1990 No;20(11):2389-93 Levy Y; Fermand JP; Brouet JC. 9. Immunophenotypes in ‘classical’ B-cell chronic lymphocytic leukemia. Correlation with normal cellular counterpart and clinical findings. Cancer 1990 Oct 1;66(8):1738-42 Baldini L; Cro L; Cortelezzi A; Calori R; Nobili L; Maiolo AT; Polli EE. 10. B-cell differentiation following autologous, conventional, or T-cell depleted bone marrow transplantation: a recapitulation of normal B-cell ontogeny. Blood 1990 Oct 1;76(8):1647-56 Small TN; Keever CA; Weiner-Fedus S; Heller G; O’Reilly RJ; Flomenberg N. 11. Phenotypic analysis of a large number of normal human bone marrow sample by flow cytometry. Blut 1990 No;61(5):271-7 Andreoni C; Rigal D; Bonnard M; Bernaud J. 12. Identification and characterization of plasma cells in normal human bone marrow by high-resolution flow cytometry. Blood 1990 Nov ;76(9):1739-47 Terstappen LW; Johnsen S; Segers-Nolten IM; Loken MR.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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