Product Name :
Mouse Anti Human CD4

Description :
| Clone Edu-2 | Isotype IgG2a | Product Type Primary Antibodies | Units 100 ug/ml | Host Mouse | Species Reactivity Human | Application Flow Cytometry Immunocytochemistry Immunohistochemistry (frozen) Immunohistochemistry (paraffin)

Background :
Edu-2 is a mouse monoclonal IgG2a antibody raised against CD4.

Source :
CD4 (Cluster of Differentiation 4) is a glycoprotein expressed on the surface of T helper cells, monocytes, macrophages, and dendritic cells. It was discovered in the late 1970s and was originally known as leu-3 and T4 (after the OKT4 monoclonal antibody that reacted with it) before being named CD4 in 1984. In humans, the CD4 protein is encoded by the CD4 gene. CD4 plays a role in the recognition of foreign antigens presented to T cells by MHC class II molecules. Furthermore, this antigen acts as a receptor for HIV-1 by binding the viral protein gp120. The CD4 antigen is present on most thymocytes and a subpopulation of peripheral blood T cells, called T helper cells (Th). In addition, CD4 is expressed on monocytes and weak on macrophages. <

Product :
Each vial contains 1 ml of 100µg/ml purified monoclonal antibody in 0.01 M sodium phosphate, 0.15 M NaCl, 0.2% BSA, 0.09% sodium azide; pH 7.3. Formulation: Each vial contains 1 ml of 100µg/ml purified monoclonal antibody in 0.01 M sodium phosphate, 0.15 M NaCl, 0.2% BSA, 0.09% sodium azide; pH 7.3.

Specificity :
CD4 (Edu-2) recognizes the CD4 antigen (a 55 kD glycoprotein).

Applications :
Flow cytometry and immunohistochemistry using frozen and paraffin embedded tissue sections. Optimal antibody dilution should be determined by titration. CD4 (Edu-2) is used in routine blood testing for CD4+ cells and CD4/CD8 ratios (e.g. HIV/AIDS patients) or as part of panels for the detection and differentiation of certain T cell leukemias.All these reagents are effectively formulated for indirect immunofluorescent staining of human blood for flow cytometric analysis using 10 µl/106 leukocytes.Staining with clone Edu-2 (CD4) monoclonal antibodies by flow cytometry analysis of normal blood cells is illustrated. Indirect staining was performed using 10 µl of the purified monoclonal antibody with RaM FITC conjugate and 100 µl blood sample. However each investigator should titer the antibody in their application to determine the optimal per-test amount.CD4 is also used in studies of functional activity of Th-cells in bacterial and viral infections, development of auto-immune diseases, transplant rejection, immune protection in response to allergens or allergenic reactivity.

Storage :
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References :
1. Carrière, D., et al., 1995, In: Leukocyte Typing V: 475-476. 2. Piatier-Tonneau, D., et al.,1995, In: Leukocyte Typing V: 476-478. 3. Kaneoka, H. et al., 1983. J. Immunol., 131: 158 4. Lanier, L.L., et al., 1986. J. Immunol., 137: 2501 5. Knowles, R.W., 1986. In: Leukocyte Typing II : 259-288; 6. E.L. Reinhert, B.F. Haynes, L.M. Nadler/ and I.D. Bernstein, eds. Springer-Verlag, New York 7. Friedrich, W., 1982. Blood, 59: 696 8. Allison, J.P. and Lanier, L.L., 1987. Ann. Rev. Immunol., 5: 503

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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