Product Name :
Mouse anti Human CD7
Description :
| Clone MG34 | Isotype IgG2a | Product Type Single-Color Reagent | Units 100 µg | Host Mouse | Species Reactivity Human | Application Flow Cytometry
Background :
Immunogen: CD7=Derived from the hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells of BALB/c mice immunized with T-acute lymphoblastic leukemia (T-ALL) cells.
Source :
Identification of human T lymphocytes in multiple stages of T cell development, including a major subset of mature peripheral T cells. CD7 antigen is often increased on T leukemic cells. The CD7 molecule is a 40,000 M.W. surface antigen that is expressed on T-Lymphoid and myeloid precursors in fetal liver and bone marrow. Synonyms: T-cell antigen CD7; GP40; T-cell leukemia antigen; TP41; Leu-9 <
Product :
Product Form: Unconjugated Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide Purification Method: Protein A/G Chromatography Concentration: See vial for concentration
Specificity :
Applications :
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2° to 8° C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20°C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. Functional Analysis: Flow Cytometry Staining
Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Room Temperature
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
1. Isolation and characterization of the genomic human CD7 gene: structural similarity with the murine Thy-1 gene. Schanberg, L.E., Fleener, D.E., Kurtzberg, J., Haynes, B.F., Kaufman, R.E.; Proc. Natl. Acad. Sci. USA , 1991 Jan 1;88(2):603-7. 2. Identification of novel B-lineage cells in human fetal bone marrow that coexpress CD7. Grumayer, E.R., Griesinger, F., Hummell, D.S., Brunning, R.D., Kersey, J.H.; Blood, 1991 Jan ; 77(1):64-8. 3. Genuine CD7 expression in acute leukemic and lymphoblastic lymphoma. Osada, H., Emi, N., Ueda, R., Seto, M., Koike, K., Suchi, T., Kojima, S., Obata, Y., Takahashi, T.; Leuk. Res. 1990;14(10):869-77. 4. Inhibition of alloresponsive naive and memory T cells by CD7 and CD25 antibodies and by cyclosporine. Akbar, A.N., Amlot, P.L., Ivory,K., Timms, A., Janossy, G.; Transplantation, 1990 No;50(5):823-9. 5. Comparsion of outcome, clinical, laboratory, and immunological features in 164 children and adults with T-ALL. Garand, R., Vannier, J.P., Bene, M.C., Faure, G., Favre, M., Bernard, A,. Leukemia,1990 No;4(11):739-44.
Related websites: https://www.medchemexpress.com/antibodies.html
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