Product Name :
Armenian Hamster anti CD27

Description :
| Clone LG.3A10 | Isotype IgG | Product Type Primary Antibodies | Units 0.1 mg | Host Armenian Hamster | Species Reactivity Human Mouse | Application ELISA Flow Cytometry Immunohistochemistry (frozen) Immunohistochemistry (paraffin) Immunoprecipitation

Background :
mAbs to murine CD27 were generated injecting a mixture of the mCD27tag expresssing clones AR0524 and AR0530; Hamster spleen cells and SP2/0 murine myeloma cells were fused.

Source :
Members of the tumor necrosis factor receptor (TNFR) family are emerging as important molecules implicated in the regulation of proliferation, differentiation and survival of T and B lymphocytes. Among these receptors is CD27, the function of which has thus far only been studied in the human system, where it amplifies the T cell proliferative response induced by TCR triggering. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a pro-apoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. <

Product :
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide. Formulation: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Specificity :
LG.3A10 and LG.8A6 see a different epitope. A combination can therefore be developed into a double determinant ELISA to detect soluble Mouse CD27. LG.3A10 cross-react with Human CD27, LG.8A6 does not.

Applications :
LG.3A10 is useful for flow cytometry, immunoprecipitation and immunohistochemistry on frozen and paraffin embedded sections . Optimal antibody dilution should be determined by titration. Recommended dilution for immunohistochemistry: 1:100. In combination with LG.8A6, it is possible to develop a double determinant ELISA to detect soluble Mouse CD27.

Storage :
The antibody may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product. Shipping Conditions: The antibody is shipped at ambient temperature.

Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References :
1. Gravestein, L.A., Nieland, J.D., Kruisbeek, A.M. and Borst, J. Int. Immunol. 7: 551-557, 1995. 2. Gravestein, L., van Ewijk, W., Ossendorp, F. and Borst, J. J. Exp. Med. 184: 675-685, 1996. 3. Hendriks, J., Gravestein, L.A., Tesselaar, K., van Lier, R.A.W., Schumacher, T.N.M., and Borst, J.Nature Immunol. 1: 433-440, 2000.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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