Product Name :
Mouse anti Human Myogenin

Description :
| Clone F12B | Isotype IgG1 | Product Type Monoclonal Antibody | Units 100 µg | Host Mouse | Species Reactivity Human Mouse Rat | Application ELISA Gel Supershift Immunofluorescence Immunohistochemistry (paraffin) Immunoprecipitation Western Blotting

Background :
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant protein containing rat myogenin amino acids 30-224 and mouse myeloma NS1 cells.

Source :
Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas. Synonyms: Myogenin, Myogenic factor 4, Myf-4 <

Product :
X1415M, clone F12B recognizes a protein of 34kDa, identified as myogenin. Its epitope localizes between aa 158-178 of myogenin. Product Form: Unconjugated Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide Purification Method: Protein A/G Chromatography Concentration: See vial for concentration

Specificity :

Applications :
This antibody can be used for ELISA, gel supershift assays, immunofluorescence, immunoprecipitation of protein-DNA complexes (2 µg/mg), Western blotting (1 µg/ml) and is the antibody of choise for immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections (1-2 µg/ml). Optimal concentration should be evaluated by serial dilutions. Functional Analysis: Western Blotting Positive Control: Rhabdomyosarcoma and Wlim’s tumors

Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References :
1. Wright WE, et al. Monoclonal antimyogenin antibodies define epitopes outside the bHLH domain where binding interferes with protein-protein and protein-DNA interaction. Dev Genet 19: 131-138 (1996). 2. Wright WE, et al. Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD. Cell 1989; 56:607. 3. Aurade F, et al. Myf5, MyoD, myogenin and MRF4 myogenic derivatives of the embryonic mesenchymal cell line C3H10T1/2 exhibit the same adult muscle phenotype. Differentiation 1994; 55:185.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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