Product Name :
Mouse anti Human NGF (Nerve Growth Factor)

Description :
| Clone AS18 | Isotype IgG1 | Product Type Monoclonal Antibody | Units 500 µg | Host Mouse | Species Reactivity Bovine Human Mouse Viper | Application Enzyme Immunoassay Functional Inhibition Immunoprecipitation

Background :
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with human recombinant nerve growth factor and mouse myeloma cells.

Source :
Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signal transduction. Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species. Recently, it has been shown that cells which do not normally respond to NGF only express low affinity receptors from transfected NGF genes but cells which normally are NGF-responsive express high affinity receptors from the same NGF sequences. This supports the hypothesis that the high affinity receptors have a unique component in addition to the subunit common to the low affinity receptors. Synonyms: NGF, hNGF, Beta-nerve growth factor, Beta-NGF, NGF, NGFB <

Product :
Product Form: Unconjugated Formulation: Provided as solution in phosphate buffered saline, no preservative Purification Method: Protein A/G Chromatography Concentration: See vial for concentration

Specificity :

Applications :
Antibody can be used for immunoprecipitation, EIA and for neutralizing NGF in functional studies. Optimal concentration should be evaluated by serial dilutions. Functional Analysis: Immunoprecipitation Positive Control: PC12 cells

Storage :
Store at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: Products are stable for one year from purchase when stored properly Shipping Conditions: Ship at ambient temperature

Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References :
1. Kitamura, T., et al., J. Cell Physiol. 1989: 140, 322. 2. Francke, U., et al., Science 1983: 222, 1248. 3. Zabel, B., et al., Proc. Natl. Acad. Sci. USA 1985: 82, 469. 4. Selby, M. et al., Mol. Cell Biol. 1987: 7, 3057.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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