Product Name :
Mouse anti Human NPDC-1

Description :
| Clone 16E4 | Isotype IgG1 | Product Type Monoclonal Antibody | Units 100 µg | Host Mouse | Species Reactivity Human Mouse Rat | Application Western Blotting

Background :
Hybridoma produced by the fusion of splenocytes from mice immunized with a synthetic peptide SLGSPVSSDPVHMS derived from human NPDC-1 protein (Gene ID: 56654) and mouse myeloma cells.

Source :
The discovery of NPDC-1 (neuronal proliferation, differentiation and control protein 1) was the result of a study of genes that were preferentially expressed in immortalized neural precursor cell lines at the onset of contact inhibition of proliferation and subsequent differentiation. It was further found that transfection of transformed cells with NPDC-1 led to increased doubling times and suppression of many characteristics of transformation. NPDC-1 is differentially expressed in brain and lung and has been shown to interact directly with the transcription factor E2F-1 as well as D-type cyclins and cdk2. NPDC-1 has been identified as a modulator of transcriptional events mediated by retinoic acid and the protein level and activity of NPDC-1 have been shown to be regulated by phosphorylation mediated proteasomal degradation. <

Product :
Product Form: Unconjugated Formulation: 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol, pH 7.5, 3mM sodium azide Purification Method: Ion-exchange chromatography Concentration: See vial for concentration

Specificity :
Monoclonal Anti-NPDC-1 (mouse IgG1 isotype) is derived from the hybridoma 16E4 produced by immunizing mice with the peptide SLGSPVSSDPVHMS (Gene ID: 56654).

Applications :
Antibody can be used for Western blotting (1:1000 dilution). Optimal concentration should be evaluated by serial dilutions. Monoclonal Anti-NPDC-1 recognizes human NPDC-1 by immunoblotting (34-40 kDa). Functional Analysis: Western Blotting Positive Control: Brain tissue lysate (mouse and rat tested, human not yet tested).

Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References :
1. Spencer, M.L., et al. ‘NPDC-1, a novel regulator of neuronal proliferation, is degraded by the ubiquitin/proteasome system through a PEST degradation motif.’ J. Biol. Chem. (2004), 279, 37069-37078 2. Evrard, C. & Rouget P. ‘Subcellular localization of neural-specific NPDC-1 protein.’ J Neurosci Res (2005), 79, 747-755 3. Sansal, I., et al. ‘NPDC-1, a regulator of neural cell proliferation and differentiation, interacts with E2F-1, reduces its binding to DNA and modulates its transcriptional activity.’ Oncogene (2000), 19, 5000-5009

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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